A Cellular Anatomy of the Normal Adult Human Prostate and Prostatic Urethra

被引：201

作者：

Henry, Gervaise H. ^{[1
]}

Malewska, Alicia ^{[1
]}

Joseph, Diya B. ^{[2
]}

Malladi, Venkat S. ^{[3
]}

Lee, Jeon ^{[3
]}

Torrealba, Jose ^{[4
]}

Mauck, Ryan J. ^{[1
]}

Gahan, Jeffrey C. ^{[1
]}

Raj, Ganesh V. ^{[1
]}

Roehrborn, Claus G. ^{[1
]}

Hon, Gary C. ^{[5
]}

MacConmara, Malcolm P. ^{[6
]}

Reese, Jeffrey C. ^{[7
]}

Hutchinson, Ryan C. ^{[1
]}

Vezina, Chad M. ^{[2
]}

Strand, Douglas W. ^{[1
]}

机构：

[1] UT Southwestern Med Ctr, Dept Urol, Dallas, TX 75390 USA

[2] Univ Wisconsin, Sch Vet Med, Dept Comparat Biosci, Madison, WI 53706 USA

[3] UT Southwestern Med Ctr, Dept Bioinformat, Dallas, TX 75390 USA

[4] UT Southwestern Med Ctr, Dept Pathol, Dallas, TX 75390 USA

[5] UT Southwestern Med Ctr, Dept Obstet & Gynecol, Cecil H & Ida Green Ctr Reprod Biol Sci, Dallas, TX 75390 USA

[6] UT Southwestern Med Ctr, Dept Surg, Dallas, TX 75390 USA

[7] Southwest Transplant Alliance, Dallas, TX 75231 USA

来源：

CELL REPORTS | 2018年 / 25卷 / 12期

关键词：

TO-LUMINAL DIFFERENTIATION; STEM-CELLS; GENE-EXPRESSION; NEUROENDOCRINE DIFFERENTIATION; ACCELERATES INITIATION; CANCER; IDENTIFICATION; INFLAMMATION; EPITHELIUM; DATABASE;

D O I：

10.1016/j.celrep.2018.11.086

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A comprehensive cellular anatomy of normal human prostate is essential for solving the cellular origins of benign prostatic hyperplasia and prostate cancer. The tools usedto analyze the contribution of individual cell types are not robust. We provide a cellular atlas of the young adult human prostate and prostatic urethra using an iterative process of single-cell RNA sequencing (scRNA-seq) and flow cytometry on similar to 98,000 cells taken from different anatomical regions. Immunohistochemistry with newly derived cell type-specific markers revealed the distribution of each epithelial and stromal cell type on whole mounts, revising our understanding of zonal anatomy. Based on discovered cell surface markers, flow cytometry antibody panels were designed to improve the purification of each cell type, with each gate confirmed by scRNA-seq. The molecular classification, anatomical distribution, and purification tools for each cell type in the human prostate create a powerful resource for experimental design in human prostate disease.

引用

页码：3530 / +

页数：18

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