Identification of a cadmium-induced gene in Mycobacterium bovis and Mycobacterium tuberculosis

被引:1
|
作者
Hotter, GS [1 ]
Wilson, T [1 ]
Collins, DM [1 ]
机构
[1] AgRes, Wallaceville Anim Res Ctr, Upper Hutt, New Zealand
关键词
Mycobacterium tuberculosis; cadmium; gene expression;
D O I
10.1111/j.1574-6968.2001.tb10707.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A 17-kDa protein (CadI) was induced by cadmium in Mycobacterium bovis and Mycobacterium tuberculosis. Comparison of the N-terminal sequence from M. bovis CadI with the annotated M. tuberculosis genome database identified Rv2641 as the encoding gene. Long and short promoter fragments from M. bovis cadI were fused to the lacZ reporter gene in pYUB76. Only the long fragment directed cadmium-inducible activity when electroporated into M. bovis. The cadI promoter has potential for both constitutive and inducible expression studies in M. bovis and M. tuberculosis. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:151 / 155
页数:5
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