Comparison of Two Nucleic Acid Amplification Assays, the Xpert MTB/RIF Assay and the Amplified Mycobacterium Tuberculosis Direct Assay, for Detection of Mycobacterium tuberculosis in Respiratory and Nonrespiratory Specimens

被引：49
|
作者：
Teo, Jeanette [1 ]
Jureen, Roland [1 ]
Chiang, Donald [1 ]
Chan, Douglas [1 ]
Lin, Raymond [1 ]
机构：
[1] Natl Univ Singapore Hosp, Dept Lab Med, Microbiol Unit, Singapore 119074, Singapore
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 10期
关键词：
RIFAMPIN RESISTANCE;
D O I：
10.1128/JCM.00211-11
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We compared the performance of the Xpert MTB/RIF assay, a new real-time tuberculosis (TB) PCR test, with that of the Amplified Mycobacterium Tuberculosis Direct (MTD) assay using 162 respiratory and nonrespiratory specimens. Based on culture as the gold standard, the overall sensitivity and specificity for all sample types for the Xpert MTB/RIF assay were 90.9 and 89%, respectively, while for the MTD assay, the overall sensitivity and specificity were 97.3 and 87.1%, respectively. A higher proportion of total equivocal results were obtained for the MTD assay, at 10.5% (17/162), while the Xpert MTB/RIF assay generated 5.5% (9/162) of invalid reads.
引用
收藏
页码：3659 / 3662
页数：4
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