Chronic Moderate Alcohol Intakes Accelerate SR-B1 Mediated Reverse Cholesterol Transport

被引：7
|
作者：
Li, Menghua [1 ,2 ,3 ]
Diao, Yan [1 ,2 ]
Liu, Ying [4 ]
Huang, Hui [1 ,2 ]
Li, Yanze [1 ,2 ]
Tan, Peizhu [1 ,2 ]
Liang, Huan [1 ,2 ,5 ]
He, Qi [1 ,2 ]
Nie, Junhui [1 ,2 ]
Dong, Xingli [1 ,2 ]
Wang, Yang [1 ,2 ]
Zhou, Lingyun [1 ,2 ]
Gao, Xu [1 ,2 ]
机构：
[1] Harbin Med Univ, Dept Biochem & Mol Biol, Harbin, Peoples R China
[2] Translat Med Ctr Northern China, Harbin, Peoples R China
[3] SIPO, Patent Off, Patent Examinat Cooperat Ctr, Mianyang, Sichuan, Peoples R China
[4] Heilongjiang Prov Hosp, Dept Gastroenterol, Harbin, Peoples R China
[5] Harbin Med Univ, Canc Hosp, Dept Clin Lab, Harbin, Peoples R China
来源：
SCIENTIFIC REPORTS | 2016年 / 6卷
基金：
中国国家自然科学基金;
关键词：
B TYPE-I; CONSUMPTION; DISEASE; RISK; DRINKING; DENSITY; MICE; STEATOSIS; PROMOTER; ENZYMES;
D O I：
10.1038/srep33032
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Cholesterol is essential for all animal life. However, a high level of cholesterol in the body is strongly associated with the progression of various severe diseases. In our study, the potential involvement of alcohol in the regulation of high density lipoprotein (HDL) receptor scavenger receptor class B and type I (SR-B1)-mediated reverse cholesterol transport was investigated. We separated male C57BL/6 mice into four diets: control, alcohol, Control + HC and alcohol + HC. The SR-B1 level and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate-high-density lipoprotein (DiI-HDL) uptake were also measured in AML12 cells and HL7702 cells treated with alcohol. The control + HC diet led to increased hepatic triglyceride and cholesterol levels while alcohol + HC led no significant change. Compared with that of the control group, the SR-B1 mRNA level was elevated by 27.1% (P < 0.05), 123.8% (P < 0.001) and 343.6% (P < 0.001) in the alcohol, control + HC and alcohol + HC groups, respectively. In AML12 and HL7702 cells, SR-B1 level and DiI-HDL uptake were repressed by SR-B1 siRNA or GW9662. However, these effects were reversed through alcohol treatment. These data suggest that a moderate amount of alcohol plays a novel role in reverse cholesterol transport, mainly mediated by PPAR. and SR-B1.
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页数：11
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