Chronic Moderate Alcohol Intakes Accelerate SR-B1 Mediated Reverse Cholesterol Transport

被引：7
|
作者：
Li, Menghua [1 ,2 ,3 ]
Diao, Yan [1 ,2 ]
Liu, Ying [4 ]
Huang, Hui [1 ,2 ]
Li, Yanze [1 ,2 ]
Tan, Peizhu [1 ,2 ]
Liang, Huan [1 ,2 ,5 ]
He, Qi [1 ,2 ]
Nie, Junhui [1 ,2 ]
Dong, Xingli [1 ,2 ]
Wang, Yang [1 ,2 ]
Zhou, Lingyun [1 ,2 ]
Gao, Xu [1 ,2 ]
机构：
[1] Harbin Med Univ, Dept Biochem & Mol Biol, Harbin, Peoples R China
[2] Translat Med Ctr Northern China, Harbin, Peoples R China
[3] SIPO, Patent Off, Patent Examinat Cooperat Ctr, Mianyang, Sichuan, Peoples R China
[4] Heilongjiang Prov Hosp, Dept Gastroenterol, Harbin, Peoples R China
[5] Harbin Med Univ, Canc Hosp, Dept Clin Lab, Harbin, Peoples R China
来源：
SCIENTIFIC REPORTS | 2016年 / 6卷
基金：
中国国家自然科学基金;
关键词：
B TYPE-I; CONSUMPTION; DISEASE; RISK; DRINKING; DENSITY; MICE; STEATOSIS; PROMOTER; ENZYMES;
D O I：
10.1038/srep33032
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Cholesterol is essential for all animal life. However, a high level of cholesterol in the body is strongly associated with the progression of various severe diseases. In our study, the potential involvement of alcohol in the regulation of high density lipoprotein (HDL) receptor scavenger receptor class B and type I (SR-B1)-mediated reverse cholesterol transport was investigated. We separated male C57BL/6 mice into four diets: control, alcohol, Control + HC and alcohol + HC. The SR-B1 level and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate-high-density lipoprotein (DiI-HDL) uptake were also measured in AML12 cells and HL7702 cells treated with alcohol. The control + HC diet led to increased hepatic triglyceride and cholesterol levels while alcohol + HC led no significant change. Compared with that of the control group, the SR-B1 mRNA level was elevated by 27.1% (P < 0.05), 123.8% (P < 0.001) and 343.6% (P < 0.001) in the alcohol, control + HC and alcohol + HC groups, respectively. In AML12 and HL7702 cells, SR-B1 level and DiI-HDL uptake were repressed by SR-B1 siRNA or GW9662. However, these effects were reversed through alcohol treatment. These data suggest that a moderate amount of alcohol plays a novel role in reverse cholesterol transport, mainly mediated by PPAR. and SR-B1.
引用
收藏
页数：11
相关论文
共 50 条
  • [1] Chronic Moderate Alcohol Intakes Accelerate SR-B1 Mediated Reverse Cholesterol Transport
    Menghua Li
    Yan Diao
    Ying Liu
    Hui Huang
    Yanze Li
    Peizhu Tan
    Huan Liang
    Qi He
    Junhui Nie
    Xingli Dong
    Yang Wang
    Lingyun Zhou
    Xu Gao
    Scientific Reports, 6
  • [2] microRNA-145 Regulates ABCA1, SR-B1, and Reverse Cholesterol Transport
    Mutharasan, R. Kannan
    Zhang, Heng
    Ardehali, Hossein
    Thaxton, C. Shad
    CIRCULATION, 2012, 126 (21)
  • [3] Into The Wormhole: Understanding SR-B1's Cholesterol Transport Functions Through Structural Studies
    Powers, Hayley R.
    Jenjak, Shawn
    Volkman, Brian
    Sahoo, Daisy
    ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2022, 42
  • [4] Into The Wormhole: Understanding SR-B1's Cholesterol Transport Functions Through Structural Studies
    Powers, Hayley R.
    Jenjak, Shawn
    Volkman, Brian
    Sahoo, Daisy
    ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2022, 42
  • [5] HEPATIC CHOLESTEROL DYSREGULATION IN SR-B1/APOE DKO MICE
    Xiong, Ting
    Yu, Pei
    Lebeau, Paul
    Austin, Richard C.
    Trigatti, Bernardo L.
    ATHEROSCLEROSIS SUPPLEMENTS, 2018, 32 : 38 - 39
  • [6] SR-B1: A Unique Multifunctional Receptor for Cholesterol Influx and Efflux
    Shen, Wen-Jun
    Azhar, Salman
    Kraemer, Fredric B.
    ANNUAL REVIEW OF PHYSIOLOGY, VOL 80, 2018, 80 : 95 - 116
  • [7] CONTRIBUTION OF CHOLESTEROL-BINDING MOTIFS IN ABCA1, ABCG1 AND SR-B1 STRUCTURE TO CELLULAR CHOLESTEROL TRANSPORT
    Dergunov, A.
    Savushkin, E.
    Dergunova, L.
    Litvinov, D.
    ATHEROSCLEROSIS, 2018, 275 : E169 - E169
  • [8] Effect of moderate alcohol consumption on parameters of reverse cholesterol transport in postmenopausal women
    Sierksma, A
    Vermunt, SHF
    Lankhuizen, IM
    van der Gaag, MS
    Scheek, LM
    Grobbee, DE
    van Tol, A
    Hendriks, HFJ
    ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 2004, 28 (04) : 662 - 666
  • [9] Novel Human Variants Of SR-B1 Impact HDL-cholesterol Clearance
    Powers, Hayley
    Sahoo, Daisy
    ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2023, 43
  • [10] Sepsis syndrome stimulates proximal tubule cholesterol synthesis and suppresses the SR-B1 cholesterol transporter
    Zager, RA
    Johnson, ACM
    Hanson, SY
    KIDNEY INTERNATIONAL, 2003, 63 (01) : 123 - 133
12345