Full-field time-resolved fluorescence tomography of small animals

被引：81
|
作者：
Venugopal, Vivek [1 ]
Chen, Jin [1 ]
Lesage, Frederic [2 ,3 ]
Intes, Xavier [1 ]
机构：
[1] Rensselaer Polytech Inst, Dept Biomed Engn, Troy, NY 12180 USA
[2] Ecole Polytech Montreal, Dept Genie Elect, Montreal, PQ H3C 3A7, Canada
[3] Ecole Polytech Montreal, Inst Genie Biomed, Montreal, PQ H3C 3A7, Canada
来源：
OPTICS LETTERS | 2010年 / 35卷 / 19期
基金：
加拿大自然科学与工程研究理事会;
关键词：
TURBID MEDIA; ILLUMINATION; LIGHT;
D O I：
10.1364/OL.35.003189
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
In this experimental investigation, we explore the feasibility of using wide-field illumination for time-resolved fluorescence molecular tomography. The performance of wide-field patterns with a time-resolved imaging platform is investigated in vitro and in a small animal model. A Monte Carlo-based forward model is employed to reconstruct fluorescence yield based on time-gated datasets. An improvement in resolution and quantification when using the time-gate data type compared to the commonly used cw data type is demonstrated in vitro. Furthermore, the feasibility of wide-field strategies for fluorescence preclinical applications is established by an accurate localization of a fluorescent inclusion implanted in the chest cavity of a murine model. (C) 2010 Optical Society of America
引用
收藏
页码：3189 / 3191
页数：3
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