Generation of a TPM1 homozygous knockout embryonic stem cell line by CRISPR/Cas9 editing

被引：5
|
作者：
Ma, Shuhong [1 ,2 ]
Xu, Qi [3 ]
Bai, Rui [1 ,2 ]
Dong, Tao [1 ,2 ]
Peng, Zhiping [4 ]
Liu, Xujie [4 ]
机构：
[1] Capital Med Univ, Anzhen Hosp, Beijing 100029, Peoples R China
[2] Beijing Inst Heart Lung & Blood Vessel Dis, Beijing 100029, Peoples R China
[3] Chinese Acad Med Sci & Peking Union Med Coll, Fuwai Hosp, Natl Ctr Cardiovasc Dis, State Key Lab Cardiovasc Dis, Beijing 100037, Peoples R China
[4] Chongqing Med Univ, Basic Med Sch, Dept Radiol, Chongqing 400016, Peoples R China
来源：
STEM CELL RESEARCH | 2021年 / 55卷
基金：
中国国家自然科学基金; 北京市自然科学基金;
关键词：
D O I：
10.1016/j.scr.2021.102470
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Alpha-Tropomyosin (TPM1) plays a crucial role in actin regulation and stability and contributes fundamental functions to heart development: without TPM1 expressing, mice embryos will die early in embryogenesis. To further identify the role of TPM1 in human cardiac development, here we generated a homozygous TPM1 knockout (TPM1(-/-)) human embryonic stem cell (hESC) line using CRISPR/Cas9-based genome editing system. The generated TPM1(-/-) hESC line maintained normal karyotype, highly expressed pluripotency markers and was able to differentiate into all three germ layers in vivo. This cell line provides a powerful tool to investigate the role of TPM1 in heart development in future.
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页数：4
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