The Mobile Phase Motion in Ascending Micellar Thin-Layer Chromatography with Normal-Phase Plates

The physical chemical characteristics (surface tension and viscosity) of micellar mobile phases based on the cationic surfactant cetylpiridinium chloride and additives of alcohols (ethanol, 1-propanol, 1-butanol, 1-pentanol) have been obtained in this work. The effect of mobile phase properties on the motion of mobile phase in the ascending mode of thin-layer chromatography (TLC) was investigated. The applicability of a quadratic relationship between the time of mobile phase motion and the distance from the solvent entry position of mobile phase and front of the mobile phase was validated for micellar TLC. It was observed that the retardation factors of hydrophilic test compounds change significantly only at low contents of the modifier in micellar mobile phase.