Estimation of genome size in Brachiaria species

被引：13

作者：

Ishigaki, Genki ^{[1
]}

Gondo, Takahiro ^{[1
]}

Ebina, Masumi ^{[2
]}

Suenaga, Kazuhiro ^{[3
]}

Akashi, Ryo ^{[1
]}

机构：

[1] Miyazaki Univ, Frontier Sci Res Ctr, Miyazaki 8892192, Japan

[2] Natl Agr & Food Res Org, Natl Inst Livestock & Grassland Sci, Nasushiobara, Tochigi, Japan

[3] Japan Int Res Ctr Agr Sci, Tsukuba, Ibaraki, Japan

来源：

GRASSLAND SCIENCE | 2010年 / 56卷 / 04期

关键词：

Brachiaria; flow cytometry; genome size; DNA; PLANTS; SEQUENCES; EVOLUTION;

D O I：

10.1111/j.1744-697X.2010.00200.x

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Genome sizes of five cultivars of four Brachiaria species were estimated by flow cytometry to evaluate their utility as breeding material. Genome sizes expressed as C values differed among these cultivars (615-1953 Mbp C-1), increasing with increased ploidy level. Genome sizes given as Cx values were larger in apomictic polyploids (651-817 Mbp Cx-1) than in sexual diploids (615 Mbp Cx-1). Additionally, genome sizes expressed as Cx values tended to decrease with increasing ploidy levels in apomictic cultivars. Our findings indicate that genome size depends on ploidy level and reproductive mode in Brachiaria. The information is useful for research on the construction of DNA libraries, genetic maps, biodiversity and evolution of Brachiaria species.

引用

页码：240 / 242

页数：3

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