Micropropagation of Drosera peltata, a tuberous sundew, by shoot tip culture

被引:16
|
作者
Kim, KS [1 ]
Jang, GW
机构
[1] Chonnam Natl Univ, Inst Agr Sci & Technol, Gwangju 500757, South Korea
[2] Chonnam Natl Univ, Dept Hort, Gwangju 500757, South Korea
[3] Korean Carnivorous Plant Inst, Gwangju 501250, South Korea
关键词
carnivorous plant; in vitro propagation; MS medium; pH; tuber formation;
D O I
10.1023/B:TICU.0000016812.66762.45
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In order to establish and optimize an in vitro micropropagation method of Drosera peltata ( a tuberous sundew), a carnivorous plant, the effects of medium type, MS medium concentration, pH and cytokinin type on shoot proliferation and tuber formation were investigated, using one month-old shoot tips. The shoot proliferation and tuber formation were most effective on 1/2 MS medium without cytokinins. The optimum pH of the media was pH 5.7. Tubers were planted in plastic pots filled with 1:1 peat moss and sand. The survival rate of the plantlets was almost 100%, and they exhibited normal development. With subculture every 12 weeks, hundreds of the plants were propagated from a single plant within a year.
引用
收藏
页码:211 / 214
页数:4
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