PCR-RFLP and sequence analysis of 12S and 16S rRNA mitochondrial genes of Grey mullets from East coast of India

被引:0
|
作者
Shekhar, M. S. [1 ]
Natarajan, M. [1 ]
Kumar, K. Vinaya [1 ]
机构
[1] Cent Inst Brackishwater Aquaculture, Genet & Biotechnol Unit, Madras 600028, Tamil Nadu, India
关键词
Gene; marine; mullet; phylogenetic; estuarine; PHYLOGENETIC-RELATIONSHIPS; MOLECULAR PHYLOGENY; MTDNA SEGMENTS; MUGILIDAE; DNA; DIVERGENCE; TELEOSTEI; EVOLUTION; MARKERS;
D O I
暂无
中图分类号
P7 [海洋学];
学科分类号
0707 ;
摘要
Present study consists PCR-RFLP and sequence analysis of mitochondrial genes (12S and 16S rRNA) on four species of Mugilidae, collected from east coast of India. Three and four restriction enzymes were found informative for 12S rRNA mitochondrial gene for Liza macrolepis and Liza rode respectively. Whereas, eight, seven and eight restriction enzymes were found informative for Liza macrolepis, Liza parsia and Liza fade respectively for 16S rRNA mitochondrial gene. Sequence analysis of 12S rRNA and 16S rRNA revealed that Liza macroplepis was distant from the other two Liza species, L. parsia and L. rode. Mugil cephalus was found to be the most distinct amongst the mullet species analyzed based on sequence analysis of 16S rRNA mitochondrial gene.
引用
收藏
页码:529 / 534
页数:6
相关论文
共 50 条
  • [21] A new PCR-RFLP method for detection of Anaplasma marginale based on 16S rRNA
    Vahid Noaman
    Parviz Shayan
    Veterinary Research Communications, 2010, 34 : 43 - 50
  • [22] A new PCR-RFLP method for detection of Anaplasma marginale based on 16S rRNA
    Noaman, Vahid
    Shayan, Parviz
    VETERINARY RESEARCH COMMUNICATIONS, 2010, 34 (01) : 43 - 50
  • [23] Phylogenetic relationships of Hynobius naevius as revealed by mitochondrial 12S and 16S rRNA genes (Amphibia: Caudata)
    Tominaga, Atsushi
    Matsui, Masafumi
    Nishikawa, Kanto
    Tanabe, Shingo
    ZOOLOGICAL SCIENCE, 2005, 22 (12) : 1434 - 1434
  • [24] Molecular phylogeny of the family Pectinidae (Mollusca: Bivalvia) based on mitochondrial 16S and 12S rRNA genes
    Barucca, M
    Olmo, E
    Schiaparelli, S
    Canapa, A
    MOLECULAR PHYLOGENETICS AND EVOLUTION, 2004, 31 (01) : 89 - 95
  • [25] Phylogenetic relationships of African microhylid frogs inferred from DNA sequences of mitochondrial 12S and 16S rRNA genes
    Loader, SP
    Gower, DJ
    Howell, KM
    Doggart, N
    Rödel, MO
    Clarke, BT
    de Sá, RO
    Cohen, BL
    Wilkinson, M
    ORGANISMS DIVERSITY & EVOLUTION, 2004, 4 (04) : 227 - 235
  • [26] Mutation analysis of the D-Loop and the 12S and 16S rRNA genes in prostate cancer
    Gomez-Zaera, M
    Abril, J
    Gonzalez, L
    Nadal, M
    Nunes, V
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2004, 1657 : 80 - 81
  • [27] Mitochondrial 12S rRNA sequence analysis in wildlife forensics
    Prakash, S
    Patole, MS
    Ghumatkar, SV
    Nandode, SK
    Shinde, BM
    Shouche, YS
    CURRENT SCIENCE, 2000, 78 (10): : 1239 - 1241
  • [28] Use of PCR-RFLP for genotyping 16S rRNA and characterizing bacteria cultured from halibut fry
    Jensen, S
    Bergh, O
    Enger, O
    Hjeltnes, B
    CANADIAN JOURNAL OF MICROBIOLOGY, 2002, 48 (05) : 379 - 386
  • [29] Molecular identification of five commercial flatfish species by PCR-RFLP analysis of a 12S rRNA gene fragment
    Comesaña, AS
    Abella, P
    Sanjuan, A
    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2003, 83 (08) : 752 - 759
  • [30] Use of PCR-RFLP for genotyping 16S rRNA and characterizing bacteria cultured from halibut fry
    Jensen, Sigmund
    Bergh, Øivind
    Enger, Øivind
    Hjeltnes, Brit
    2002, National Research Council of Canada (48)