Physico-chemical and biological properties of a nano-hydroxyapatite powder synthesized at room temperature

Objective. - The aim of this work was to synthesize and to characterize chemically and biologically (in vitro and in vivo) a nano-sized hydroxyapatite (nHA) Materials and methods. - Wet chemical precipitation at room temperature was performed, then chemical structure was explored using transmission electron microscopy. X-ray diffraction and Fourier transformed infrared spectroscopy In vitro biological characterization was done using MG63 osteoblastic cell line cultured onto the material, and characterization was done for morphology (scanning electron microscopy), viability (live/dead assay) and proliferation (MTT test) Finally, nHA powder was tested in vivo in a study involving C57 Black mice for bone repair in a calvarial bone critical sized defect. Results - Morphological, physico-chemical and cristallographic analyses revealed specific features of hydrox.,,apatite Biological in vitro experiments revealed high affinity and proliferative ability of MG63 cells cultured onto the material In vivo study displayed that in this model, the material allowed to repair bone continuity alter I month healing Discussion and conclusions - The different ways of nHA synthesis are discussed regarding the potential application of the material. The obtained material should find applications in bone tissue engineering experiments (C) 2010 Elsevier Masson SAS. All rights reserved