Phenome analysis of root development in Arabidopsis

Mutant lines covering all Arabidopsis genes allow us to pursue systematic functional genomics. A comprehensive phenotypic description, called phenome, is highly sought after in the profiling of -omics. We previously selected 4,000 transposon-insertional lines with transpson insertion in their gene-coding regions, systematically observed and recorded the visible phenotype of aerial portion of each line (ecotype Nossen) (Kuromori et al. 2006). In this paper, we tried to observe root phenotypes of seedling in these transposon-insertional lines with transpson insertion in their gene-coding regions. For example, we measured the length of main root and the length of the root-hair. We totally observed root-phenotypes of 1817 lines, and identified seven phenotypes in roots. We also ordered alleles from Salk-knock-out lines (ecotype Col-0) to make sure that one line showed the similar phenotype among them.