Detection of cabbage yellows fungus Fusarium oxysporum f. sp conglutinans in soil by PCR and real-time PCR

被引：8

作者：

Kashiwa, Takeshi ^{[1
,2
,3
]}

Inami, Keigo ^{[3
,4
]}

Teraoka, Tohru ^{[3
]}

Komatsu, Ken ^{[3
]}

Arie, Tsutomu ^{[3
]}

机构：

[1] Tokyo Univ Agr & Technol, United Grad Sch Agr Sci, Fuchu, Tokyo 1838509, Japan

[2] RIKEN, CSRS, Chem Biol Res Grp, Wako, Saitama 3510198, Japan

[3] Tokyo Univ Agr & Technol, Grad Sch Agr, Plant Pathol Lab, Fuchu, Tokyo 1838509, Japan

[4] Bridgestone Corp, Div Cent Res, Res Dept 1, Tokyo 1878531, Japan

来源：

JOURNAL OF GENERAL PLANT PATHOLOGY | 2016年 / 82卷 / 05期

关键词：

Cabbage; eDNA; Fusarium oxysporum; Real-time PCR; RESISTANCE; DNA;

D O I：

10.1007/s10327-016-0668-5

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

For the specific detection of Fusarium oxysporum f. sp. conglutinans (Foc), we designed a primer set for PCR and a primer-probe set for real-time PCR based on its endopolygalacturonase gene sequence. Using the primer set, we could distinguish Foc from other pathogenic forms and nonpathogenic isolates of F. oxysporum. Moreover, we detected the fungus by real-time PCR using DNA isolated from soil. Sensitivity of real-time PCR was improved 10- to 10,000-fold by adding bovine serum albumin or by performing pre-PCR. Our method can detect Foc in the soil at a density that causes only slight yellows symptom.

引用

页码：240 / 247

页数：8

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