Adrenergic receptors and secretory responses of the rat submandibular salivary gland after periodic incisor reduction

The lower and upper incisors of female rats were repeatedly reduced every 48 hr for 21 days. A marked enlargement of the submandibular glands was observed at the end of this period. One day after the final reduction, dose-dependent curves to phenylephrine and isoproterenol were obtained in relation to salivary flow rates. Secretory responses, expressed as mg/gland, showed that the dose-response curve to the al-adrenomimetic drug was not modified by treatment while that for isoproterenol was shifted to the right of the control. When the responses were expressed as mu g of saliva/mg of wet tissue, the dose-response curve to both agonists was shifted to the right in the incisor-reduced group. (Activation of alpha(2)-adrenergic receptors by clonidine did not inhibit the responses to phenylephrine in the incisor-reduced rats.) Radioligand binding assays of alpha(1)-, beta- and alpha(2)-receptors did not show differences between control and experimental glands in terms of densities (B-max) or affinities (K-d) The lack of correlation between the decrease in alpha(2)- and beta-mediated responses and the radioligand bindings suggests that postreceptor mechanisms are involved in the diminished secretory responses of the rat submandibular gland after periodic reduction or amputation of incisors. (C) 1998 Elsevier Science Ltd. All rights reserved.