Structural investigation of a polysaccharide (Fr. II) isolated from the aqueous extract of an edible mushroom, Pleurotus sajor-caju

被引:65
|
作者
Pramanik, M [1 ]
Mondal, S [1 ]
Chakraborty, I [1 ]
Rout, D [1 ]
Islam, SS [1 ]
机构
[1] Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India
关键词
Pleurotus sajor-caju; mushroom polysaccharide; structure; NMR experiments;
D O I
10.1016/j.carres.2004.12.032
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A water-soluble polysaccharide, (Fr. II) isolated from aqueous extract of an edible mushroom Pleurotus sajor-caja, was found to consist Of D-glucose, D-galactose, and D-mannose in a molar proportion of 1: 1: 1. Compositional analysis, methylation analysis, periodate oxidation study, partial hydrolysis, and NMR experiments (H-1, C-13, 2D-COSY, TOCSY, NOESY, HSQC, and HMBC) revealed the presence of the following repeating unit in the polysaccharide: -> 6)-alpha-D-Galp-(1 -> 4)-alpha-D-Glcp-(1 -> 2 up arrow l beta-D-Manp (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:629 / 636
页数:8
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