Integration of DNA bio-gates and duplex-specific nuclease signal amplification: towards electrochemiluminescence detection of survivin mRNA

被引:33
|
作者
Wang, Jing [1 ,2 ,3 ]
Li, Xiang-Ling [1 ,2 ]
Zhang, Jia-Dong [1 ,2 ]
Hao, Nan [1 ,2 ]
Xu, Jing-Juan [1 ,2 ]
Chen, Hong-Yuan [1 ,2 ]
机构
[1] Nanjing Univ, State Key Lab Analyt Chem Life Sci, Sch Chem & Chem Engn, Nanjing 210093, Jiangsu, Peoples R China
[2] Nanjing Univ, Collaborat Innovat Ctr Chem Life Sci, Sch Chem & Chem Engn, Nanjing 210093, Jiangsu, Peoples R China
[3] China Pharmaceut Univ, Sch Sci, Dept Analyt Chem, Nanjing 210009, Jiangsu, Peoples R China
关键词
MESOPOROUS SILICA NANOPARTICLES; CANCER-CELLS; ELECTROGENERATED CHEMILUMINESCENCE; FLUORESCENCE DETECTION; APOPTOSIS; RELEASE; EXPRESSION; MICRORNAS; DELIVERY; PROTEIN;
D O I
10.1039/c5cc03693c
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Using the DNA bio-gate and duplex-specific nuclease assisted target recycling, a facile electrochemiluminescence assay was developed for the sensitive detection of survivin mRNA.
引用
收藏
页码:11673 / 11676
页数:4
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