Inulin affects iron dialyzability from FeSO4 and FeEDTA solutions but does not alter Fe uptake by Caco-2 cells

被引：13
|
作者：
Laparra, Jose Moises [1 ]
Tako, Elad [1 ]
Glahn, Raymond P. [2 ]
Miller, Dennis D. [1 ]
机构：
[1] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA
[2] USDA, ARS, Plant Soil & Nutr Lab, Ithaca, NY 14853 USA
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2008年 / 56卷 / 08期
关键词：
prebiotic; inulin; iron bioavailability; iron uptake; ferritin; Caco-2; cells;
D O I：
10.1021/jf073470m
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The in vitro effects of inulin on the fluxes of Fe (F-Fe) and uptake by Caco-2 cells from FeSO4 and FeEDTA were evaluated. Cell ferritin formation was used as a measure of Fe uptake. Mitochondrial (MTT test) and lysosomal activities were monitored as biomarkers of the changes of cellular metabolism. Changes in mRNA expression of Fe transporters, DMT1 and Dcytb, were evaluated. Inulin decreased dialyzability and F-Fe from FeSO4 solution, suggesting a mineral binding effect, but increased those from FeEDTA. Cultures exposed to FeEDTA solutions exhibited higher ferritin values and MTT conversion percentages. Regardless of Fe source, cell Fe uptake and mRNA expression of Fe transporters were similar with or without inulin, suggesting that inulin did not impair Fe uptake. These observations might indicate a faster cellular Fe internalization from FeEDTA solutions. From a physiological perspective, the decreased F-Fe from FeSO4 might be reflected in a decreased Fe uptake.
引用
收藏
页码：2846 / 2851
页数：6
相关论文
共 40 条
  • [21] Carbohydrate fractions from cooked fish promote iron uptake by Caco-2 cells
    Glahn, RP
    Huh, EC
    Hotchkiss, A
    FASEB JOURNAL, 2004, 18 (05): : A767 - A767
  • [22] Carbohydrate fractions from cooked fish promote iron uptake by Caco-2 cells
    Huh, EC
    Hotchkiss, A
    Brouillette, J
    Glahn, RP
    JOURNAL OF NUTRITION, 2004, 134 (07): : 1681 - 1689
  • [23] Isolated Glycosaminoglycans from Cooked Haddock Enhance Nonheme Iron Uptake by Caco-2 Cells
    Laparra, Jose Moises
    Tako, Elad
    Glahn, Raymond P.
    Miller, Dennis D.
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (21) : 10346 - 10351
  • [24] Peptides isolated from in vitro digests of milk enhance iron uptake by Caco-2 cells
    Argyri, K.
    Miller, D.
    Glahn, R.
    Kapsokefalou, M.
    ANNALS OF NUTRITION AND METABOLISM, 2007, 51 : 209 - 209
  • [25] Mechanisms of Iron Uptake from Ferric Phosphate Nanoparticles in Human Intestinal Caco-2 Cells
    Perfecto, Antonio
    Elgy, Christine
    Valsami-Jones, Eugenia
    Sharp, Paul
    Hilty, Florentine
    Fairweather-Tait, Susan
    NUTRIENTS, 2017, 9 (04):
  • [26] Peptides isolated from in vitro digests of milk enhance iron uptake by caco-2 cells
    Argyri, Konstantina
    Miller, Dennis D.
    Glahn, Raymond P.
    Zhu, Le
    Kapsokefalou, Maria
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (25) : 10221 - 10225
  • [27] Dietary Factors Modulate Iron Uptake in Caco-2 Cells from an Iron Ingot Used as a Home Fortificant to Prevent Iron Deficiency
    Rodriguez-Ramiro, Ildefonso
    Perfecto, Antonio
    Fairweather-Tait, Susan J.
    NUTRIENTS, 2017, 9 (09):
  • [28] Supplemental inulin does not enhance iron bioavailability to Caco-2 cells from milk- or soy-based, probiotic-containing, yogurts but incubation at 37 °C does
    Laparra, Jose Moises
    Tako, Elad
    Glahn, Raymond P.
    Miller, Dennis D.
    FOOD CHEMISTRY, 2008, 109 (01) : 122 - 128
  • [29] Alginate Inhibits Iron Absorption from Ferrous Gluconate in a Randomized Controlled Trial and Reduces Iron Uptake into Caco-2 Cells
    Wawer, Anna A.
    Harvey, Linda J.
    Dainty, Jack R.
    Perez-Moral, Natalia
    Sharp, Paul
    Fairweather-Tait, Susan J.
    PLOS ONE, 2014, 9 (11):
  • [30] Uptake and retention of calcium, iron, and zinc from raw legumes and the effect of cooking on lentils in Caco-2 cells
    Viadel, Blanca
    Barbera, Reyes
    Farre, Rosaura
    NUTRITION RESEARCH, 2006, 26 (11) : 591 - 596
1234