Complete genome sequencing and genomic characterization of two Escherichia coli strains co-producing MCR-1 and NDM-1 from bloodstream infection

被引:23
|
作者
Zheng, Beiwen [1 ]
Yu, Xiao [1 ]
Xu, Hao [1 ]
Guo, Lihua [1 ]
Zhang, Jing [1 ,2 ]
Huang, Chen [1 ]
Shen, Ping [1 ]
Jiang, Xiawei [3 ]
Xiao, Yonghong [1 ]
Li, Lanjuan [1 ]
机构
[1] Zhejiang Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, State Key Lab Diag & Treatment Infect Dis, Affiliated Hosp 1,Coll Med, Hangzhou, Zhejiang, Peoples R China
[2] Zhejiang Univ, Coll Med, Affiliated Hosp 1, Dept Resp Dis, Hangzhou, Zhejiang, Peoples R China
[3] Zhejiang Chinese Med Univ, Coll Basic Med Sci, Hangzhou, Zhejiang, Peoples R China
来源
SCIENTIFIC REPORTS | 2017年 / 7卷
基金
中国国家自然科学基金;
关键词
RESISTANCE GENE MCR-1; PLASMID; COEXISTENCE; EMERGENCE; MEAT;
D O I
10.1038/s41598-017-18273-2
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We previously described the discovery of two Escherichia coli isolates (EC1002 and EC2474) co-harbouring mcr-1 and bla(NDM-1) genes, which were recovered from bloodstream infection in China. More importantly, these antibiotic resistance genes were located on different plasmids and signaling the potential spread of pandrug-resistant bacteria. Here, the complete genome sequences of both isolates were determined using Pacbio RS II and Illumina HiSeq2000 systems. The genome of EC1002 consists of a 5,177,501 base pair chromosome and four circular plasmids, while the genome of EC2474 consists of a 5,013,813 base pair chromosome and three plasmids. The plasmid replicon type of pEC1002_NDM and pEC2474_NDM were identified as IncA/C2 and IncF, respectively. The genetic environment of bla(NDM-1) in this study was similar to bla(NDM)-carrying plasmids detected in China, although the overall nucleotide identity and query coverage were variable. The plasmid replicon type of pEC1002_MCR and pEC2474_MCR were identified as IncI2 and IncHI2, respectively. Two different genetic strategies for mcr-1 gene spread were observed in this study and bla(NDM-1) genes were also found transferred by two different mobile genetic elements in two plasmids. The findings of this study further support that the diversified transfer mechanisms of bla(NDM-1) and mcr-1 present in Enterobacteriaceae.
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页数:7
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