Differentiation and Variability in the Rhizosphere and Endosphere Microbiomes of Healthy and Diseased Cotton (Gossypium sp.)

被引:12
|
作者
Shi, Yingwu [1 ,2 ,3 ,4 ]
Yang, Hongmei [1 ,2 ,3 ]
Chu, Ming [1 ,2 ,3 ]
Niu, Xinxiang [3 ,4 ]
Wang, Ning [1 ,2 ]
Lin, Qing [1 ,2 ]
Lou, Kai [1 ,2 ]
Zuo, Changgeng [1 ,2 ,5 ]
Wang, Jingyi [1 ,2 ,5 ]
Zou, Qiang [1 ,2 ,5 ]
Zhang, Yumeng [1 ,2 ,5 ]
机构
[1] Xinjiang Acad Agr Sci, Inst Microbiol, Urumqi, Peoples R China
[2] Xinjiang Lab Special Environm Microbiol, Urumqi, Peoples R China
[3] Minist Agr & Countryside, Key Lab Agr Environm Northwest Oasis, Urumqi, Peoples R China
[4] Xinjiang Acad Agr Sci, Inst Soil Fertilizer & Agr Water Conservat, Urumqi, Peoples R China
[5] Xinjiang Univ, Coll Life Sci & Technol, Urumqi, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
endophytic microbiome; rhizosphere microbiome; healthy and diseased; cotton; diversity; Illumina MiSeq; BACTERIAL COMMUNITIES; ENDOPHYTIC COLONIZATION; SOIL; PLANT; MANAGEMENT; DIVERSITY; HIRSUTUM; DYNAMICS; ROOTS;
D O I
10.3389/fmicb.2021.765269
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The plant microbiome is a key determinant of health and productivity. However, it is still difficult to understand the structural composition of the bacterial and fungal microbiomes of diseased and healthy plants, especially the spatial dynamics and phylogenies of endophytic and rhizosphere microbial communities. We studied the differentiation and variability in the rhizosphere and endosphere microbiomes of healthy and diseased cotton from north and south of the Tianshan Mountains using the methods of PCR-based high-throughput sequencing and real-time quantitative PCR. The endophytic and rhizosphere bacterial abundances in the diseased plants were greater than those of healthy plants. The numbers of endophytic and rhizosphere fungi associated with diseased plants were greater than those associated healthy plants (p < 0.05). Endophytic and rhizosphere bacteria did not share common OTUs. The dominant rhizosphere bacteria were Proteobacteria (29.70%), Acidobacteria (23.14%), Gemmatimonadetes (15.17%), Actinobacteria (8.31%), Chloroflexi (7.99%), and Bacteroidetes (5.15%). The dominant rhizosphere fungi were Ascomycota (83.52%), Mortierellomycota (7.67%), Basidiomycota (2.13%), Chytridiomycota (0.39%), and Olpidiomycota (0.08%). The distribution of dominant bacteria in different cotton rhizosphere soils and roots differed, with the dominant bacteria Pseudomonas (15.54%) and Pantoea (9.19%), and the dominant fungi Alternaria (16.15%) and Cephalotrichum (9.10%) being present in the greatest numbers. At sampling points in different ecological regions, the total numbers of cotton endophytic and rhizosphere microbiome OTUs from southern to northern Xinjiang showed an increasing trend. There were significant differences in the composition and diversity of rhizosphere microbes and endophytes during the entire cotton growth period and in representative ecological regions (p < 0.01), whereas rhizosphere microbes and endophytes showed no significant differences among the four growth periods and in representative ecological regions. RB41, H16, Nitrospira, and Sphingomonas play important roles in the microbial ecology of cotton rhizosphere soil. Pseudomonas accounted for a large proportion of the microbes in the cotton rhizosphere soil. This study provides an in-depth understanding of the complex microbial composition and diversity associated with cotton north and south of the Tianshan Mountains.
引用
收藏
页数:12
相关论文
共 50 条
  • [41] Effect of Methyl Jasmonate on Phytoalexins Biosynthesis and Induced Disease Resistance to Fusarium oxysporum f. sp. Vasinfectum in Cotton (Gossypium hirsutum L.)
    Konan, Yao Kouakou Franois
    Kouassi, Kan Modeste
    Kouakou, Kouakou Laurent
    Koffi, Edmond
    Kouassi, Koffi Nazaire
    Sekou, Diabate
    Kone, Mongomake
    Kouakou, Tanoh Hilaire
    INTERNATIONAL JOURNAL OF AGRONOMY, 2014, 2014
  • [42] Inoculum densities of Fusarium oxysporum f. sp. vasinfectum and Meloidogyne incognita in relation to the development of Fusarium wilt and the phenology of cotton plants (Gossypium hirsutum)
    DeVay, JE
    Gutierrez, AP
    Pullman, GS
    Wakeman, RJ
    Garber, RH
    Jeffers, DP
    Smith, SN
    Goodell, PB
    Roberts, PA
    PHYTOPATHOLOGY, 1997, 87 (03) : 341 - 346
  • [43] Dynamic responses to Fusarium wilt (Fusarium oxysporum f. sp. vasinfectum) race 4 in two introgressed populations of Upland cotton (Gossypium hirsutum)
    Jinfa Zhang
    Abdelraheem Abdelraheem
    Yi Zhu
    Terry A. Wheeler
    Jane K. Dever
    Jiwen Yu
    Yuzhen Shi
    Youlu Yuan
    Tom Wedegaertner
    Euphytica, 2021, 217
  • [44] Bacillus and Streptomyces VOC suppression of Fusarium oxysporum f. sp. vasinfectum race 4 (Fov4) virulence on pima cotton (Gossypium barbadense)
    Murty, L.
    Shim, W. B.
    PHYTOPATHOLOGY, 2021, 111 (10) : 166 - 166
  • [45] Morphophysiological and biochemical responses of cotton (Gossypium barbadense L.) to nano zinc (ZnO-NPs) and Azospirillum sp. under water deficit stress conditions
    El-Beltagi, Hossam S.
    El-Waraky, Essam Abdelaziz
    Almutairi, Hayfa Habes
    Al-Daej, Mohammed I.
    El-Nady, Mohamed Fathi
    Shehata, Wael F.
    Belal, Elsayed B.
    El-Mogy, Mohamed M.
    El-Mehasseb, Ibrahim
    Metwaly, Metwaly Mahfouz Salem
    JOURNAL OF PLANT NUTRITION, 2025, 48 (02) : 326 - 344
  • [46] Bacterial Populations in Subgingival Plaque Under Healthy and Diseased Conditions: Genomic Insights into Oral Adaptation Strategies by Lactobacillus sp. Strain DISK7
    Urvashi
    Sharma, Deepika
    Sharma, Shikha
    Pal, Vijay
    Lal, Rup
    Patil, Prabhu
    Grover, Vishakha
    Korpole, Suresh
    INDIAN JOURNAL OF MICROBIOLOGY, 2020, 60 (01) : 78 - 86
  • [47] Bacterial Populations in Subgingival Plaque Under Healthy and Diseased Conditions: Genomic Insights into Oral Adaptation Strategies by Lactobacillus sp. Strain DISK7
    Deepika Urvashi
    Shikha Sharma
    Vijay Sharma
    Rup Pal
    Prabhu Lal
    Vishakha Patil
    Suresh Grover
    Indian Journal of Microbiology, 2020, 60 : 78 - 86
  • [48] Genome sequencing and functional annotation of Bacillus sp. strain BS-Z15 isolated from cotton rhizosphere soil having antagonistic activity against Verticillium dahliae
    Chen, Zhong-Yi
    Abuduaini, Xieerwanimu
    Mamat, Nuramina
    Yang, Qi-Lin
    Wu, Meng-Jun
    Lin, Xin-Rui
    Wang, Ru
    Lin, Rong-Rong
    Zeng, Wei-Jun
    Ning, Huan-Chen
    Zhao, He-Ping
    Li, Jin-Yu
    Zhao, Hui-Xin
    ARCHIVES OF MICROBIOLOGY, 2021, 203 (04) : 1565 - 1575
  • [49] Genome sequencing and functional annotation of Bacillus sp. strain BS-Z15 isolated from cotton rhizosphere soil having antagonistic activity against Verticillium dahliae
    Zhong-Yi Chen
    Xieerwanimu Abuduaini
    Nuramina Mamat
    Qi-Lin Yang
    Meng-Jun Wu
    Xin-Rui Lin
    Ru Wang
    Rong-Rong Lin
    Wei-Jun Zeng
    Huan-Chen Ning
    He-Ping Zhao
    Jin-Yu Li
    Hui-Xin Zhao
    Archives of Microbiology, 2021, 203 : 1565 - 1575
  • [50] Sources, selection and breeding of Fusarium wilt (Fusarium oxysporum f. sp. vasinfectum) race 4 (FOV4) resistance in Upland (Gossypium hirsutum L.) cotton
    Mauricio Ulloa
    Robert B. Hutmacher
    TariLee Schramm
    Margaret L. Ellis
    Robert Nichols
    Philip A. Roberts
    Steven D. Wright
    Euphytica, 2020, 216