Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2

被引：3
|
作者：
Encabo, Hector Huerga [1 ]
Ulferts, Rachel [2 ]
Sharma, Aneesh [1 ]
Beale, Rupert [2 ]
Bonnet, Dominique [1 ]
机构：
[1] Francis Crick Inst, Hematopoiet Stem Cell Lab, London NW1 1AT, England
[2] Francis Crick Inst, Cell Biol Infect Lab, London NW1 1AT, England
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 04期
基金：
英国医学研究理事会;
关键词：
Cell Biology; Cell isolation; Cell-based Assays; Immunology; Microbiology; Microscopy; Stem Cells;
D O I：
10.1016/j.xpro.2021.100903
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord, or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-qPCR and colony forming unit assay. For complete details on the use and execution of this protocol, please refer to Huerga Encabo et al. (2021)
引用
收藏
页数：17
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