Human visceral afferent recordings: preliminary report

被引:38
|
作者
Peiris, Madusha [1 ]
Bulmer, David C. [1 ]
Baker, Mark D. [1 ]
Boundouki, George [1 ]
Sinha, Sidhartha [1 ]
Hobson, Anthony [1 ]
Lee, Kevin [2 ]
Aziz, Qasim [1 ]
Knowles, Charles H. [1 ]
机构
[1] Queen Mary Univ London, Barts & London Sch Med & Dent, Blizard Inst Cellular & Mol Sci, Ctr Digest Dis,Neurogastroenterol Grp, London, England
[2] GlaxoSmithKline, Immunoinflammat CEDD, Epinova DPU, Stevenage, Herts, England
关键词
FUNCTIONAL GASTROINTESTINAL DISORDERS; IRRITABLE-BOWEL-SYNDROME; VAGUS NERVE; IN-VITRO; PAIN; RECEPTOR; MUSCLE; COLON; SENSITIZATION; MUCOSA;
D O I
10.1136/gut.2010.221820
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background Conditions characterised by chronic visceral pain represent a significant healthcare burden with limited treatment options. While animal models have provided insights into potential mechanisms of visceral nociception and identified candidate drug targets, these have not translated into successful treatments in humans. Objective To develop an in vitro afferent nerve preparation using surgically excised freshly isolated human colon and vermiform appendix-mesentery tissues. Methods Non-inflamed appendix (n=18) and colon (n=9) were collected from patients undergoing right and left hemicolectomy. Electrophysiological recordings were made from mesenteric nerves and the tissue stimulated chemically and mechanically. Results Ongoing neuronal activity was sparse and where units occurred peak firing rates were: colon (2.0 +/- 0.4 spikes/s, n=4) and appendix (2.4 +/- 0.6 spikes/s, n=9). Afferent nerves innervating the appendix responded with a significant increase in activity following stimulation with inflammatory mediators (73 +/- 10.6 vs 3.0 +/- 0.3 spikes/s, n=6, p<0.001, inflammatory mediator vs baseline) and capsaicin (63 +/- 15.8 vs 2 +/- 0.3 spikes/s, n=3, p<0.001, capsaicin vs buffer). Afferent nerves innervating the colon responded with increased activity to blunt probing of the serosal surface. Conclusions This first-in-human study demonstrates afferent nerve recordings from human gut tissue ex vivo and shows that tissue may be stimulated both chemically and mechanically to study neuronal responses. Collectively, the results provide preliminary evidence to validate this in vitro human tissue model as one that may aid future disease mechanistic studies and candidate drug testing.
引用
收藏
页码:204 / 208
页数:5
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