One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device

被引:53
|
作者
Aveyard, Jenny
Mehrabi, Maryam
Cossins, Andrew
Braven, Helen
Wilson, Robert [1 ]
机构
[1] Univ Liverpool, Dept Chem, Liverpool L69 7ZD, Merseyside, England
[2] Univ Liverpool, Dept Biol Sci, Liverpool L69 7ZD, Merseyside, England
[3] BBInternational, Cardiff CF14 5DX, Wales
关键词
D O I
10.1039/b708859k
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Specific PCR products are detected with an antibody-free lateral-flow device by sandwiching them between reporter oligonucleotides covalently attached to gold nanoparticles (GNPs) and capture oligonucleotides covalently attached to a nitrocellulose chromatographic strip.
引用
收藏
页码:4251 / 4253
页数:3
相关论文
共 50 条
  • [41] Development and characterization of a capillary-flow microfluidic device for nucleic acid detection
    Shengquan Jin
    Minhui Dai
    Fei He
    Yuhong Wang
    Bang-Ce Ye
    Sam R. Nugen
    Microsystem Technologies, 2012, 18 : 731 - 737
  • [42] One step lateral flow immunoassay for detection of Ecstasy in human urine.
    Tao, J
    DelosSantos, N
    Dou, C
    CLINICAL CHEMISTRY, 2002, 48 (06) : A43 - A44
  • [43] Development of a One-Step Lateral Flow Immunoassay for Rapid Detection of Icariin
    Qu, Huihua
    Qu, Baoping
    Cheng, Jinjun
    Zhang, Yue
    Zeng, Wenhao
    Wang, Qingguo
    Kong, Hui
    Zhao, Yan
    CURRENT PHARMACEUTICAL ANALYSIS, 2018, 14 (02) : 101 - 107
  • [44] Highly sensitive visual detection of nucleic acid based on a universal strand exchange amplification coupled with lateral flow assay strip
    Wang, Xiaokun
    Wang, Xiudan
    Shi, Chao
    Ma, Cuiping
    Chen, Lingxin
    TALANTA, 2020, 216
  • [45] Gold nanoparticle-based lateral flow biosensor for rapid visual detection of Leishmania-specific DNA amplification products
    Toubanaki, Dimitra K.
    Athanasiou, Evita
    Karagouni, Evdokia
    JOURNAL OF MICROBIOLOGICAL METHODS, 2016, 127 : 51 - 58
  • [46] Density and structure of DNA immobilised on gold nanoparticles affect sensitivity in nucleic acid detection
    Fukuzumi, Nanami
    Hirao, Gen
    Ogawa, Atsushi
    Asahi, Tsuyoshi
    Maeda, Mizuo
    Zako, Tamotsu
    SCIENTIFIC REPORTS, 2025, 15 (01):
  • [47] Nucleic acid-based electrochemical biosensor for detection of influenza B by gold nanoparticles
    Yahyavi, Isar
    Edalat, Fahime
    Pirbonyeh, Neda
    Letafati, Arash
    Sattarahmady, Naghmeh
    Heli, Hossein
    Moattari, Afagh
    JOURNAL OF MOLECULAR RECOGNITION, 2024, 37 (02)
  • [48] Visual Detection of Chicken Adulteration Based on a Lateral Flow Strip-PCR Strategy
    Xu, Haoyi
    Lan, Hangzhen
    Pan, Daodong
    Xu, Junfeng
    Wang, Xiaofu
    FOODS, 2022, 11 (15)
  • [49] A PCR-based lateral flow assay for the detection of Turkey ingredient in food products
    Zhao, Liangjuan
    Wang, Kaidi
    Yan, Chuncai
    Xiao, Jing
    Wu, Hong
    Zhang, Hongwei
    Zhang, Xia
    Zhang, Can
    Hu, Yaxi
    Lu, Xiaonan
    Zheng, Wenjie
    FOOD CONTROL, 2020, 107
  • [50] A fluorometric lateral flow assay for visual detection of nucleic acids using a digital camera readout
    Maria Magiati
    Areti Sevastou
    Despina P. Kalogianni
    Microchimica Acta, 2018, 185