Effect of surface potential of reverse micelle on enzyme-substrate complex formation

The influence of the electrostatic potential of reverse micelle on the enzyme-substrate complex formation have been studied within the framework of the encounter complex (EC) formation theory. Reverse micelles have a multiple-factor effect on encapsulated substances and on mechanisms of their interaction. The action of individual constituents on the overall micellar effect was analyzed by means of Brownian dynamics simulation. The effects of surface potential in charged and neutral reverse micelles, of the size and of the ionic strength of micellar core on the probability to form the reaction complex between serine protease (trypsin) and specific substrate N alpha-benZOYI-L-arginine ethyl ester was examined. It was shown that negative potential of micelle increases the probability of EC formation and the positive potential decreases it. Orientation of substrate in electrostatic field of micelle depends on the value and the sign of surface potential. (C) 2007 Elsevier B.V. All rights reserved.