Isolation, sequencing and characterization of cluster genes involved in the biosynthesis and utilization of the siderophore of marine fish pathogen Vibrio alginolyticus

被引：26
|
作者：
Wang, Qiyao [1 ]
Liu, Qin [1 ]
Ma, Yue [1 ]
Zhou, Lingyun [1 ]
Zhang, Yuanxing [1 ]
机构：
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
ARCHIVES OF MICROBIOLOGY | 2007年 / 188卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Vibrio alginolyticus; siderophore; biosynthesis; uptake; cluster gene; plasposon;
D O I：
10.1007/s00203-007-0261-6
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
In fish pathogen Vibrio alginolyticus MVP01, the isolated 11-gene cluster consisted of two divergently transcribed, Fe3+ and ferric uptake regulator (Fur) regulated operons, pvsABCDE and psuA-pvuABCDE, sharing high similarity with that related to siderophore biosynthesis and transportation locus in V. parahaemolyticus. Siderophore biosynthesis or utilization was blocked when pvsA and pvsD of the pvsABCDE operon or pvuA, pvuB and pvuE of the psuA-pvuABCDE operon was single-gene in-frame mutated, demonstrating their essential roles for siderophore biosynthesis or utilization in V. alginolyticus MVP01. Addition of the purified siderophore restored the cell growth in siderophore biosynthesis mutants, but not in siderophore uptake mutants.
引用
收藏
页码：433 / 439
页数：7
相关论文
共 50 条
  • [41] GENES INVOLVED IN FLAVANOID BIOSYNTHESIS IN PETUNIA - ISOLATION AND CHARACTERIZATION OF THE GENE(S) FOR CHALCONE SYNTHASE, THE KEY ENZYME OF FLAVONOID SYNTHESIS
    KOES, RE
    SPELT, CE
    VELTKAMP, E
    MOL, JNM
    ACTA BOTANICA NEERLANDICA, 1987, 36 (3-4): : 314 - 314
  • [42] De novo sequencing, assembly, and characterization of Asparagus racemosus transcriptome and analysis of expression profile of genes involved in the flavonoid biosynthesis pathway
    Malik, Chanchal
    Dwivedi, Sudhanshu
    Rabuma, Tilahun
    Kumar, Ravinder
    Singh, Nitesh
    Kumar, Anil
    Yogi, Rajesh
    Chhokar, Vinod
    FRONTIERS IN GENETICS, 2023, 14
  • [43] Cloning and sequencing of clustered genes involved in fatty acid biosynthesis from the docosahexaenoic acid-producing bacterium, Vibrio marinus strain MP-1
    Morita, N
    Ueno, A
    Tamaka, M
    Ohgiya, S
    Hoshino, T
    Kawasaki, K
    Yumoto, I
    Ishizaki, K
    Okuyama, H
    BIOTECHNOLOGY LETTERS, 1999, 21 (07) : 641 - 646
  • [44] Cloning and sequencing of clustered genes involved in fatty acid biosynthesis from the docosahexaenoic acid-producing bacterium, Vibrio marinus strain MP-1
    Noaki Morita
    Akio Ueno
    Mika Tamaka
    Satoru Ohgiya
    Tamotsu Hoshino
    Kosei Kawasaki
    Isao Yumoto
    Kozo Ishizaki
    Hidetoshi Okuyama
    Biotechnology Letters, 1999, 21 : 641 - 646
  • [45] Isolation and characterization of pathogenic Vibrio harveyi (V-carchariae) from the farmed marine cobia fish Rachycentron canadum L. with gastroenteritis syndrome
    Liu, PC
    Lin, JY
    Chuang, WH
    Lee, KK
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2004, 20 (05): : 495 - 499
  • [46] Isolation and Characterization of Pathogenic Vibrio harveyi(V. carchariae) From the Farmed Marine Cobia Fish Rachycentron canadum L. with Gastroenteritis Syndrome
    Ping-Chung Liu
    Ji-Yang Lin
    Wen-Hsiao Chuang
    Kuo-Kau Lee
    World Journal of Microbiology and Biotechnology, 2004, 20 : 495 - 499
  • [47] Isolation of transposon mutants from Azospirillum brasilense Yu62 and characterization of genes involved in indole-3-acetic acid biosynthesis
    Xie, BE
    Xu, K
    Zhao, HX
    Chen, SF
    FEMS MICROBIOLOGY LETTERS, 2005, 248 (01) : 57 - 63
  • [48] Isolation of clustered genes that are notably homologous to the eicosapentaenoic acid biosynthesis gene cluster from the docosahexaenoic acid-producing bacterium Vibrio marinus strain MP-1
    Tanaka, M
    Ueno, A
    Kawasaki, K
    Yumoto, I
    Ohgiya, S
    Hoshino, T
    Ishizaki, K
    Okuyama, H
    Morita, N
    BIOTECHNOLOGY LETTERS, 1999, 21 (11) : 939 - 945
  • [49] Isolation of clustered genes that are notably homologous to the eicosapentaenoic acid biosynthesis gene cluster from the docosahexaenoic acid-producing bacterium Vibrio marinus strain MP-1
    Mika Tanaka
    Akio Ueno
    Kosei Kawasaki
    Isao Yumoto
    Satoru Ohgiya
    Tamotsu Hoshino
    Kozo Ishizaki
    Hidetoshi Okuyama
    Naoki Morita
    Biotechnology Letters, 1999, 21 : 939 - 945
  • [50] Isolation and characterization of two genes, waaC (rfaC) and waaF (rfaF), involved in Pseudomonas aeruginosa serotype O5 inner-core biosynthesis
    deKievit, TR
    Lam, JS
    JOURNAL OF BACTERIOLOGY, 1997, 179 (11) : 3451 - 3457
12345