Isolation, sequencing and characterization of cluster genes involved in the biosynthesis and utilization of the siderophore of marine fish pathogen Vibrio alginolyticus

被引：26
|
作者：
Wang, Qiyao [1 ]
Liu, Qin [1 ]
Ma, Yue [1 ]
Zhou, Lingyun [1 ]
Zhang, Yuanxing [1 ]
机构：
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
ARCHIVES OF MICROBIOLOGY | 2007年 / 188卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Vibrio alginolyticus; siderophore; biosynthesis; uptake; cluster gene; plasposon;
D O I：
10.1007/s00203-007-0261-6
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
In fish pathogen Vibrio alginolyticus MVP01, the isolated 11-gene cluster consisted of two divergently transcribed, Fe3+ and ferric uptake regulator (Fur) regulated operons, pvsABCDE and psuA-pvuABCDE, sharing high similarity with that related to siderophore biosynthesis and transportation locus in V. parahaemolyticus. Siderophore biosynthesis or utilization was blocked when pvsA and pvsD of the pvsABCDE operon or pvuA, pvuB and pvuE of the psuA-pvuABCDE operon was single-gene in-frame mutated, demonstrating their essential roles for siderophore biosynthesis or utilization in V. alginolyticus MVP01. Addition of the purified siderophore restored the cell growth in siderophore biosynthesis mutants, but not in siderophore uptake mutants.
引用
收藏
页码：433 / 439
页数：7
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