Isolation of renal proximal tubular brush-border membranes

被引：105
|
作者：
Biber, Juerg [1 ]
Stieger, Bruno [2 ]
Stange, Gerti [1 ]
Murer, Heini [1 ]
机构：
[1] Univ Zurich, Inst Physiol, CH-8057 Zurich, Switzerland
[2] Univ Zurich Hosp, Dept Med, Div Clin Pharmacol & Toxicol, CH-8091 Zurich, Switzerland
来源：
NATURE PROTOCOLS | 2007年 / 2卷 / 06期
关键词：
D O I：
10.1038/nprot.2007.156
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This protocol describes a method for the isolation and purification of renal proximal tubular brush-border membranes in high yield and high purity. Based on a different reactivity of the brush-border membrane compared to other cellular membranes with divalent cations, such as Mg2+, purified membrane vesicles can be obtained after a few differential centrifugation steps (within approximately 3 h) that are suitable for in vitro studies, such as transport experiments or protein and lipid analysis.
引用
收藏
页码：1356 / 1359
页数：4
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