Spatiotemporal patterns of postsynaptic density (PSD)-95 expression after rat spinal cord injury

被引:15
|
作者
Cheng, C. [1 ,2 ]
Gao, S. [2 ]
Zhao, J. [3 ]
Niu, S. [1 ]
Chen, M. [2 ]
Li, X. [2 ]
Qin, J. [2 ]
Shi, S. [2 ]
Guo, Z. [1 ]
Shen, A. [1 ]
机构
[1] Nantong Univ, Jiangsu Prov Key Lab Neuroregenerat, Nantong 226001, Jiangsu, Peoples R China
[2] Nantong Univ, Former Nantong Med Coll, Dept Microbiol & Immunol, Nantong 226001, Peoples R China
[3] Nantong Univ, Affiliated Hosp, Dept Orthopaed, Nantong 226001, Peoples R China
关键词
apoptosis; neuronal nitric oxide synthase; postsynaptic density-95; rat; real-time polymerase chain reaction; spinal cord;
D O I
10.1111/j.1365-2990.2007.00917.x
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Aims: Postsynaptic density (PSD)-95 is a scaffolding protein linking the N-methyl-D-aspartate receptor with neuronal nitric oxide synthase (nNOS), which contributes to many physiological and pathological actions. We here investigated whether PSD-95 was involved in the secondary response following spinal cord injury (SCI). Methods: Spinal cord contusion (SCC) and spinal cord transection (SCT) models at thoracic (T) segment 9 (T-9) were established in adults rats. Real-time polymerase chain reaction, Western blot, immunohistochemistry and immunofluorescence were used to detect the temporal profile and spatial distribution of PSD-95 after SCI. The association between PSD-95 and nNOS in the injured cords was also assessed by coimmmunoprecipation and double immunofluorescent staining. Results: The mRNA and protein for PSD-95 expression were significantly increased at 2 h or 8 h, and then gradually declined to the baseline level, ultimately up-regulated again from 5 days to 7 days for its mRNA level and at 7 days or 14 days for its protein level after either SCC or SCT. PSD-95 immunoreactivity was found in neurones, oligodendrocytes and synaptic puncta of spinal cord tissues within 5 mm from the lesion site. Importantly, injury-induced expression of PSD-95 was colabelled by active caspase-3 (apoptotic marker), Tau-1 (the marker for pathological oligodendrocytes) and nNOS. Conclusions: Accompanied by the spatio-temporal changes for PSD-95 expression, the association between PSD-95 and nNOS undergoes substantial alteration after SCI. These two molecules are likely to form a complex on apoptotic neurones and pathological oligodendrocytes, which may in turn be involved in the secondary response after SCI.
引用
收藏
页码:340 / 356
页数:17
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