Efficient mass production of purified recombinant porcine IL-2 with baculovirus/insect cell culture gene expression system

被引：0

作者：

Inumaru, S ^{[1
]}

Suegami, S ^{[1
]}

Kokuho, T ^{[1
]}

Yamamoto, M ^{[1
]}

Nishikawa, S ^{[1
]}

Watanabe, S ^{[1
]}

Kubota, T ^{[1
]}

机构：

[1] Natl Inst Anim Hlth, Tsukuba, Ibaraki 305, Japan

来源：

PROCEEDINGS OF THE INTERNATIONAL CYTOKINE SOCIETY ANNUAL MEETING | 2003年

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To develop an efficient mass production system of purified cytokines, we attempted to use a bioreactor and produced porcine interleukin-2 (poIL-2). The poIL-2 recombinant baculovirus infected insect cell line SF21AE was incubated in the bioreactor with 6 L of serum free medium for 72 hrs. This culture fluid contained 84 mg/L (purity: 65%) of recombinant poIL-2 (rpoIL-2). Three Litres of the fluid was filtered with 300kDa ultra-filter to remove virus particles etc. and concentrated by 10kDa ultra-filtration. The rpoIL-2 in the concentrate was precipitated between 65% and 90% saturation of ammonium sulfate. The precipitate was dissolved and dialyzed against PBS. Finally, 15.5 ml of the rpoIL-2 containing fraction (5.4 mg/ml, total: 84 mg, recovery: 33%) was prepared. Purity was 94.2%.

引用

页码：133 / 137

页数：5

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