Cell size as a tool to predict the production of recombinant protein by the insect-cell baculovirus expression system

被引：34
|
作者：
Palomares, LA
Pedroza, JC
Ramírez, OT
机构：
[1] Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Bioingn, Cuernavaca 62191, Morelos, Mexico
[2] Univ Autonoma Estado Morelos, Ctr Invest Biotecnol, Mexico City, DF, Mexico
来源：
BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 05期
关键词：
baculovirus; culture monitoring; insect cell size; metabolism; recombinant protein;
D O I：
10.1023/A:1005688417525
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The increase of Sf9 cell diameter after infection with a recombinant baculovirus encoding VP8 protein of rotavirus can be used to predict culture productivity. A direct proportional correlation between the increase in cell size and VP8 concentration was obtained when manipulating selected medium components. Only yeast extract increased (38%) VP8 concentration, while fetal bovine serum increased (55%) the maximum cell concentration. An inexpensive and simplified culture media can thus be designed without detriment to protein yields.
引用
收藏
页码：359 / 364
页数：6
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