Analysis of forensic casework samples by Precision ID Ancestry panel - manual and automated Ampliseq workflow

被引：2

作者：

Al-Dosari, Waad Rashed ^{[1
,2
]}

Al-Binali, Ibrahim Abdullah ^{[1
]}

Pydi, Satya Sudheer ^{[3
]}

Goodwin, William ^{[2
]}

机构：

[1] Minist Interior, Forens Lab Dept, Doha, Qatar

[2] Univ Cent Lancashire, Sch Forens & Appl Sci, Preston, Lancs, England

[3] Sedeer Med, Doha, Qatar

来源：

FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES | 2019年 / 7卷 / 01期

关键词：

Precision ID ancestry panel; MPS; Qatar;

D O I：

10.1016/j.fsigss.2019.10.187

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Precision ID Ancestry panel from ThermoFisher contains 165 SNP markers. In this study we compared library construction using the automated workflow with the Ion Chef and a manual workflow. Casework samples that were collected between 2005 and 2018 in the State of Qatar where used for the study and the samples varied from routine stains to challenging samples that yielded full and partial STR profiles. One hundred and forty-three samples were processed: sixteen using both workflows. Full and partial profiles were seen with both methods. The assay was sensitive; one sample with only 0.12 ng of input DNA having a full profile. No significant differences were seen between the two workflows. However, the automated workflow saved considerable hands-on lab time and reduced the potential for pipetting errors.

引用

页码：816 / 817

页数：2

共 50 条

[21] Genetic resolution of applied biosystems™ precision ID Ancestry panel for seven Asian populations
Lee, Ji Hyun
Cho, Sohee
Kim, Moon-Young
Shin, Dong Hoon
Rakha, Allah
Shinde, Vasant
Lee, Soong Deok
LEGAL MEDICINE, 2018, 34 : 41 - 47
[22] Ancestry prediction efficiency of the software GenoGeographer using a z-score method and the ancestry informative markers in the Precision ID Ancestry Panel
Mogensen, Helle S.
Tvedebrink, Torben
Borsting, Claus
Pereira, Vania
Morling, Niels
FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2020, 44
[23] Applications and Performance of Precision ID GlobalFiler NGS STR, Identity, and Ancestry Panels in Forensic Genetics
Matute, Sharlize Pedroza
Iyavoo, Sasitaran
GENES, 2024, 15 (09)
[24] Implementation of a semi-automated processing system for DNA profiling of forensic casework samples
Vollack, Kai
Haak, Barbara
Schwenzer, Ralph
Pflug, Werner
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES, 2008, 1 (01) : 83 - 85
[25] RETRACTION: Analysis of Uyghur and Kazakh populations using the Precision ID Ancestry Panel (Retraction of Vol 43, art no 102144, 2019)
Simayijiang, H.
Bosting, C.
Tvedebrink, T.
Morling, N.
FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2023, 66
[26] w Frequencies of the precision ID ancestry panel markers in Basques using the Ion Torrent PGM™ platform
Garcia, O.
Ajuriagerra, J. A.
Alday, A.
Alonso, S.
Perez, J. A.
Soto, A.
Uriarte, I.
Yurrebaso, I.
FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2017, 31 : E1 - E4
[27] Evaluation of an Automated DNA Extraction Method from FFPE Samples for the Ion AmpliSeq Cancer Panel 2.0 Assay
Popa, A.
Anderson, P.
Surve, D. A.
Sabato, M.
Ferreira-Gonzalez, A.
Dumur, C. I.
JOURNAL OF MOLECULAR DIAGNOSTICS, 2015, 17 (06): : 830 - 831
[28] Hypervariable mitochondrial DNA sequences analysis of various samples in a forensic casework.
Brignon, E
Pfitzinger, H
PROGRESS IN FORENSIC GENETICS 7, 1998, 1167 : 142 - 144
[29] AUTOMATED SHORT TANDEM REPEAT (STR) ANALYSIS IN FORENSIC CASEWORK - A STRATEGY FOR THE FUTURE
GILL, P
KIMPTON, CP
URQUHART, A
OLDROYD, N
MILLICAN, ES
WATSON, SK
DOWNES, TJ
ELECTROPHORESIS, 1995, 16 (09) : 1543 - 1552
[30] Automated processing of forensic casework samples using robotic workstations equipped with nondisposable tips: Contamination prevention
Fregeau, Chantal J.
Lett, C. Marc
Elliott, Jim
Yensen, Craig
Fourney, Ron M.
JOURNAL OF FORENSIC SCIENCES, 2008, 53 (03) : 632 - 651

← 1 2 3 4 5 →