κ-Carrageenan as a carrier in affinity precipitation of yeast alcohol dehydrogenase

被引：8
|
作者：
Mondal, K [1 ]
Roy, I [1 ]
Gupta, MN [1 ]
机构：
[1] Indian Inst Technol, Dept Chem, New Delhi 110016, India
来源：
PROTEIN EXPRESSION AND PURIFICATION | 2003年 / 32卷 / 01期
关键词：
affinity precipitation; alcohol dehydrogenase; kappa-Carrageenan; reversibly soluble-insoluble polymers; protein purification;
D O I：
10.1016/S1046-5928(03)00224-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
kappa-Carrageenan is a non-toxic polymer from seaweeds, which becomes reversibly insoluble upon the addition of K+. Its conjugate with the dye, Cibacron Blue 3GA, was used to purify alcohol dehydrogenase from crude yeast extract by affinity precipitation. Response surface methodology was used to optimize conditions for affinity precipitation of the enzyme with the polymer-dye conjugate. Recovery of 58% of the enzyme activity with 13.6-fold purification was obtained. (C) 2003 Elsevier Inc. All rights reserved.
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页码：151 / 160
页数：10
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