Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv

被引：4
|
作者：
Rhee, KH
Lee, KS
Priyadarshi, A
Kim, EE
Hwang, KY [1 ]
机构：
[1] Korea Univ, Div Biotechnol & Genet Engn, Seoul 136701, South Korea
[2] Korea Inst Sci & Technol, Div Life Sci, Biomed Res Ctr, Seoul 136791, South Korea
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2005年 / 61卷
关键词：
D O I：
10.1107/S1744309105034330
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Fatty acid-CoA racemase plays an important role in the beta-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P3(1) or P3(2), with unit-cell parameters a = b = 109.56, c = 147.97 angstrom. The asymmetric unit contains six monomers, corresponding to a V-M value of 2.15 angstrom (3) Da(-1). A complete native data set has been collected at 2.7 angstrom resolution using a synchrotron-radiation source.
引用
收藏
页码：1017 / 1019
页数：3
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