Effect of culture medium composition on the activity of extracellular lectins of Lentinus edodes

被引:13
|
作者
Tsivileva, OM
Nikitina, VE
Garibova, LV
机构
[1] Russian Acad Sci, Inst Biochem & Physiol Plants & Microorg, Saratov 410015, Russia
[2] Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119899, Russia
关键词
Nitrogen; Culture Condition; Nitrogen Source; Culture Liquid; Culture Medium Composition;
D O I
10.1007/s10438-005-0030-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The time course of lectin production in culture liquid of the basidial fungus Lentinus edodes strain F-249 in different media under submerged culture conditions was studied. The activity of agglutinins depended on the ratio between carbon and nitrogen sources and the pH of the culture medium. The lectin activity in culture medium was maximal when the fungus was grown in a medium containing L-arabinose as a source of carbon and L-asparagine as a source of nitrogen (C : N ratio, (9.5-12) : 1) on day 15-18 of culturing at pH 8.0-9.0.
引用
收藏
页码:174 / 176
页数:3
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