Combined Cross-Linked Enzyme Aggregates as Biocatalysts

被引：75

作者：

Xu, Meng-Qiu ^{[1
,2
]}

Wang, Shuang-Shuang ^{[3
]}

Li, Li-Na ^{[1
,2
]}

Gao, Jian ^{[3
]}

Zhang, Ye-Wang ^{[1
,2
,3
]}

机构：

[1] Jiangsu Univ, Sch Pharm, United Pharmaceut Inst, Zhenjiang 212013, Peoples R China

[2] Jiangsu Univ, Shandong Tianzhilvye Biotechnol Co Ltd, Zhenjiang 212013, Peoples R China

[3] Qinzhou Univ, Coll Petr & Chem Engn, Qinzhou 535011, Peoples R China

来源：

CATALYSTS | 2018年 / 8卷 / 10期

关键词：

biocatalysis; Combi-CLEAs; cascade reactions; immobilization; MAGNETIC FE3O4-AT-SIO2 NANOPARTICLES; MESOPOROUS SILICA NANOPARTICLES; NANOSTRUCTURED LIPID CARRIERS; PENICILLIN-G ACYLASE; COVALENT IMMOBILIZATION; OXIDE NANOPARTICLES; GLUCOSE-OXIDASE; FREE COIMMOBILIZATION; VERSATILE PEROXIDASE; CASCADE REACTIONS;

D O I：

10.3390/catal8100460

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Enzymes are efficient biocatalysts providing an important tool in many industrial biocatalytic processes. Currently, the immobilized enzymes prepared by the cross-linked enzyme aggregates (CLEAs) have drawn much attention due to their simple preparation and high catalytic efficiency. Combined cross-linked enzyme aggregates (combi-CLEAs) including multiple enzymes have significant advantages for practical applications. In this review, the conditions or factors for the preparation of combi-CLEAs such as the proportion of enzymes, the type of cross-linker, and coupling temperature were discussed based on the reaction mechanism. The recent applications of combi-CLEAs were also reviewed.

引用

页数：20

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