Quantitative butyrylcholinesterase activity detection by surface-enhanced Raman spectroscopy

被引：37

作者：

Nechaeva, Natalia ^{[1
]}

Prokopkina, Taisiya ^{[2
]}

Makhaeva, Galina ^{[3
]}

Rudakova, Elena ^{[3
]}

Boltneva, Natalia ^{[3
]}

Dishovsky, Christophor ^{[4
]}

Eremenko, Arkadiy ^{[1
]}

Kurochkin, Ilya ^{[1
,2
]}

机构：

[1] RAS, Emanuel Inst Biochem Phys, Moscow, Russia

[2] Lomonosov Moscow State Univ, Dept Chem, Moscow, Russia

[3] RAS, Inst Physiol Act Cpds, Chernogolovka, Russia

[4] Mil Med Acad, Sofia, Bulgaria

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2018年 / 259卷

基金：

俄罗斯科学基金会; 俄罗斯基础研究基金会;

关键词：

Butyrylcholinesterase; Thiocholine; Butyrylthiocholine; Raman spectroscopy; Silver SERS-substrate; PLS regression; SERUM BUTYRYLCHOLINESTERASE; GENETIC-VARIANTS; ACETYLCHOLINESTERASE; RISK; KINETICS;

D O I：

10.1016/j.snb.2017.11.174

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The butyrylcholinesterase activity in human blood is an important marker of healthiness. Either level of this enzyme - increased or otherwise - can indicate disease or intoxication. The most popular technique to assess the cholinesterase activity is the detection of thiocholine, a product of enzymatic hydrolysis of butyrylthiocholine. In this work, Ag-paste was used as SERS-substrate and a new technique was created for thiocholine detection. As explained below, the designed technique facilitates also determination of butyrylcholinesterase (BChE) activity both in the buffer and in spike solution with human plasma. Ellman's assay was used as a reference method, a good correlation between spectrophotometric detection and Raman detection was shown. (c) 2017 Elsevier B.V. All rights reserved.

引用

页码：75 / 82

页数：8

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