THE VERIFICATION OF DIFFERENTIALLY EXPRESSED GENES RELATED TO FEATHER COLOUR IN QUAIL EMBRYOS BY RT-qPCR BASED ON RNA-seq

被引：2

作者：

Hu, Yunqi ^{[1
]}

Zhang, Xiaohui ^{[1
,2
]}

Pang, Youzhi ^{[1
,2
]}

Qi, Yanxia ^{[1
,2
]}

Wang, Qiankun ^{[1
]}

Zhao, Yiwei ^{[1
]}

Yuan, Zhiwen ^{[1
]}

Wang, Tao ^{[1
]}

Huo, Linke ^{[1
]}

Ren, Shiwei ^{[1
]}

机构：

[1] Henan Univ Sci & Technol, Coll Anim Sci, Luoyang 471003, Henan, Peoples R China

[2] Luoyang Key Lab Anim Genet & Breeding, Luoyang 471003, Henan, Peoples R China

来源：

COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES | 2022年 / 75卷 / 08期

关键词：

quail; RT-qPCR; gene expression; RNA-seq; feather colour; JAPANESE-QUAIL; CATHEPSIN-D; MUTATIONS;

D O I：

10.7546/CRABS.2022.08.06

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Many differentially expressed genes associated with feather colour in quail embryos can be screened using RNA-seq technology. The aim of this study was to verify the accuracy of RNA-seq using RT-qPCR. The transcriptome of embryonic skin tissues of quails were sequenced by Illumina HiSeq TM2000 sequencing platform. As a result, 2512 differentially expressed genes were found. Among them, 950 differentially expressed genes were up-regulated in the skin tissues of white-feather quail embryos, and 1562 differentially expressed genes were down-regulated. The candidate genes were analyzed by RT-qPCR method to verify the accuracy and reliability of the RNA-seq. The results showed that the relative expression levels of ASIP and DDC in white-feather quails were significantly increased, while HOXD1, CTSD, KRT2, MBD2 and MT1 were significantly decreased, which was consistent with the results of RNA-seq. The validation results of the candidate genes can also provide some ideas to explain the difference in feather colour between maroon-feather quails and white-feather quails.

引用

页码：1138 / 1145

页数：8

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