Affinity chromatography of porcine pepsin A using quinolin-8-ol as ligand

被引：5

作者：

Novotná, L

Hruby, M

Benes, MJ

Kucerová, Z

机构：

[1] Charles Univ, Fac Med 1, Inst Pathophysiol, Prague 12853 2, Czech Republic

[2] Acad Sci Czech Republ, Inst Macromol Chem, CR-16206 Prague, Czech Republic

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1084卷 / 1-2期

关键词：

porcine pepsin A; affinity chromatography; quinolin-8-ol;

D O I：

10.1016/j.chroma.2004.10.058

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Stationary phase containing quinolin-8-ol immobilized on macroporous methacrylate support for the affinity chromatography of porcine pepsin A is described. Optimized chromatographic conditions for separation of porcine pepsin A on this stationary phase were found investigating the influence of pH, concentration, ionic strength and chemical composition of the used mobile phases. The stationary phase shows a good reproducibility of chromatographic analyses (relative standard deviation, +/- 2%), a high recovery (ca. 93%) and a satisfactory capacity (13 mg pepsin A/1 mL stationary phase) for porcine pepsin A. The obtained findings confirm the applicability of affinity chromatography on the stationary phase with immobilized quinolin-8-ol to the isolation and determination of porcine pepsin A. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：108 / 112

页数：5

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