Development of Enzyme-Linked Immunosorbent Assay for Analysis of Total Aflatoxins Based on Monoclonal Antibody Reactive with Aflatoxins B1, B2, G1 and G2

被引：10
|
作者：
Yamasaki, Tomomi [1 ,2 ,7 ]
Miyake, Shiro [2 ,3 ,4 ]
Sato, Natsuki [5 ]
Hirakawa, Yuki [1 ,2 ]
Iwasa, Seiji [6 ]
Narita, Hiroshi [1 ]
Watanabe, Takaho [5 ]
机构：
[1] Kyoto Womens Univ, Higashiyama Ku, Kyoto 6058501, Japan
[2] Adv Sci Technol & Management Res Inst Kyoto, Shimogyo Ku, Kyoto 6008813, Japan
[3] HORIBA Ltd, Minami Ku, Kyoto 6018510, Japan
[4] Azabu Univ, Sagamihara, Kanagawa 2525201, Japan
[5] Food & Drug Safety Ctr Hadano, Hadano, Kanagawa 2578523, Japan
[6] Toyohashi Univ Technol, Toyohashi, Aichi 4418580, Japan
[7] Osaka Inst Publ Hlth, Higashinari Ku, Osaka 5370025, Japan
来源：
FOOD HYGIENE AND SAFETY SCIENCE | 2018年 / 59卷 / 05期
关键词：
aflatoxin; total aflatoxin; immunoassay; ELISA; monoclonal antibody; SURFACE-PLASMON RESONANCE; EXPOSURE ASSESSMENT; ELISA; IMMUNOSENSOR; MYCOTOXINS; TOXICOLOGY; OUTBREAK;
D O I：
10.3358/shokueishi.59.200
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed for the determination of total amount of aflatoxin B-1, B-2, G(1) and G(2) (AFB(1), AFB(2), AFG(1) and AFG(2)), using a mouse monoclonal antibody that shows similar reactivity to each of these AFs. The working range of the developed dc-ELISA was 50-230 pg/mL for AFB(1), 50-270 pg/mL for AFB(2), 60-390 pg/mL for AFG, and 65-700 pg/mL for AFG(2). The recovery of AFs from spiked roasted peanuts was 98%. Further, when 4 samples actually contaminated with AFB(1), AFB(2), AFG(1) and AFG(2) were examined, the results of dc-ELISA were highly correlated with the values assigned by the Food Analysis Performance Assessment Scheme. The developed dc-ELISA appears to be suitable for the determination of total AFs at concentrations around the maximum permitted level (10 mu g/kg for all foods) in Japan.
引用
收藏
页码：200 / 205
页数：6
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