Glucocorticoid Receptor Contributes to Electroacupuncture-Induced Analgesia by Inhibiting Nav1.7 Expression in Rats With Inflammatory Pain Induced by Complete Freund's Adjuvant

被引:7
|
作者
Luo, Huiying [1 ]
Zhang, Yidan [2 ,3 ]
Zhang, Jingjing [2 ,3 ]
Shao, Jinping [2 ]
Ren, Xiuhua [2 ]
Zang, Weidong [2 ]
Cao, Jing [2 ,3 ]
Xu, Bo [1 ]
机构
[1] Gen Hosp Southern Theatre Command PLA, Dept Anesthesiol, Guangzhou 510010, Guangdong, Peoples R China
[2] Zhengzhou Univ, Sch Basic Med Sci, Dept Human Anat, Zhengzhou 450001, Henan, Peoples R China
[3] Zhengzhou Univ, Neurosci Res Inst, Acad Med Sci, Zhengzhou, Peoples R China
来源
NEUROMODULATION | 2022年 / 25卷 / 08期
基金
中国国家自然科学基金;
关键词
Dorsal root ganglion; electroacupuncture; glucocorticoid receptors; inflammatory pain; Nav1; 7; PITUITARY-ADRENAL AXIS; PERIPHERAL-NERVE INJURY; GATED SODIUM-CHANNELS; SPINAL MICROGLIA; NEUROPATHIC PAIN; UP-REGULATION; MODEL; ACUPUNCTURE; HYPERALGESIA; ACTIVATION;
D O I
10.1111/ner.13499
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Background While electroacupuncture (EA) has been used traditionally for the treatment of chronic pain, its analgesic mechanisms have not been fully clarified. We observed in an earlier study that EA could reverse inflammatory pain and suppress high Nav1.7 expression. However, the molecular mechanism underlying Nav1.7 expression regulation is unclear. In this study, we studied the relationship between the glucocorticoid receptor (GR) and Nav1.7 and the role of these molecules in EA analgesia. Materials and Methods In this study, we established an inflammatory pain model by intraplantar injection of complete Freund's adjuvant (CFA) in rats. EA stimulation was applied to the ipsilateral "Huantiao" (GB30) and "Zusanli" (ST36) acupoints in the rat model. Western blotting, real-time polymerase chain reaction, immunostaining, intrathecal injection, and chromatin immunoprecipitation (ChIP) assay were performed to determine whether the sodium channel protein Nav1.7 plays a role in CFA-induced pain and whether GR regulates Nav1.7 expression during analgesia following EA stimulation. Results EA application significantly decreased the paw withdrawal threshold thresholds and thermal paw withdrawal latency and suppressed GR and Nav1.7 expression in the dorsal root ganglion. Moreover, treatment with a GR sense oligonucleotide (OND) markedly reversed these alterations. In contrast, treatment with a GR antisense OND along with EA application exerted a better analgesic effect, which was accompanied by the suppression of Nav1.7 and GR protein expression. The ChIP assay showed that the binding activity of GR to the Nav1.7 promoter was enhanced in CFA injected rats and suppressed in EA-treated rats. Conclusions The present study demonstrated that EA exerted anti-hyperalgesic effects by inhibiting GR expression, which led to Nav1.7 expression modulation in the rat model of CFA-induced inflammatory pain.
引用
收藏
页码:1393 / 1402
页数:10
相关论文
共 50 条
  • [21] Anti-inflammatory effect of stevioside abates Freund's complete adjuvant (FCA)-induced adjuvant arthritis in rats
    Alavala, Sateesh
    Nalban, Nasiruddin
    Sangaraju, Rajendra
    Kuncha, Madhusudana
    Jerald, Mahesh Kumar
    Kilari, Eswar Kumar
    Sistla, Ramakrishna
    INFLAMMOPHARMACOLOGY, 2020, 28 (06) : 1579 - 1597
  • [22] Anti-inflammatory effect of stevioside abates Freund’s complete adjuvant (FCA)-induced adjuvant arthritis in rats
    Sateesh Alavala
    Nasiruddin Nalban
    Rajendra Sangaraju
    Madhusudana Kuncha
    Mahesh Kumar Jerald
    Eswar Kumar Kilari
    Ramakrishna Sistla
    Inflammopharmacology, 2020, 28 : 1579 - 1597
  • [23] Ectopic expression of Nav1.7 in spinal dorsal horn neurons induced by NGF contributes to neuropathic pain in a mouse spinal cord injury model
    Fu, Yan
    Sun, Liting
    Zhu, Fengting
    Xia, Wei
    Wen, Ting
    Xia, Ruilong
    Yu, Xin
    Xu, Dan
    Peng, Changgeng
    FRONTIERS IN MOLECULAR NEUROSCIENCE, 2023, 16
  • [24] Fragile X mental retardation protein-regulated proinflammatory cytokine expression in the spinal cord contributes to the pathogenesis of inflammatory pain induced by complete Freund's adjuvant
    Yang, Yixin
    Zhao, Jinsong
    Li, Yunze
    Li, Xiangyao
    Chen, Xiaowei
    Feng, Zhiying
    JOURNAL OF NEUROCHEMISTRY, 2021, 159 (03) : 512 - 524
  • [25] Nuclear receptor 4A1 facilitates complete Freund's adjuvant-induced inflammatory pain in rats by promoting ferroptosis in spinal glial cells
    Deng, Yifan
    Xuan, Ruoheng
    Qiu, Zhuolin
    Xiang, Ping
    Guo, Yue
    Xu, Lejia
    Zhang, Xiaohan
    Mai, Haiyan
    Li, Xiang
    BRAIN BEHAVIOR AND IMMUNITY, 2025, 125 : 92 - 109
  • [26] Anti-Inflammatory Effects of BoNT/A Against Complete Freund's Adjuvant-Induced Arthritis Pain in Rats: Transcriptome Analysis
    Li, Xinhe
    Ye, Yinshuang
    Zhou, Wenwen
    Shi, Qilin
    Wang, Lin
    Li, Tieshan
    FRONTIERS IN PHARMACOLOGY, 2021, 12
  • [27] Synergistic antinociceptive effects of N-methyl-D-aspartate receptor antagonist and electroacupuncture in the complete Freund's adjuvant-induced pain model
    Jang, Ji-Yeon
    Kim, Ha-Neui
    Koo, Sung-Tae
    Shin, Hwa-Kyoung
    Choe, Eun-Sang
    Choi, Byung-Tae
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2011, 28 (04) : 669 - 675
  • [28] Disabling phosphorylation at the homer ligand of the metabotropic glutamate receptor 5 alleviates complete Freund's adjuvant-induced inflammatory pain
    Luo, Limin
    Huang, Min
    Zhang, Yu
    Wang, Wenying
    Ma, Xiaqing
    Shi, Haibo
    Worley, Paul F.
    Kim, Dong Kwan
    Fedorovich, Sergei, V
    Jiang, Wei
    Xu, Tao
    NEUROPHARMACOLOGY, 2020, 170
  • [29] Spinal GABA is involved in the role of EA in relieving complete Freund's adjuvant induced inflammatory pain in mice
    Li W. M
    Li N
    Cui K. M
    Gu Q. B
    Schwarz, W.
    JOURNAL OF NEUROCHEMISTRY, 2006, 98 : 32 - 32
  • [30] Continuous infusion of substance P inhibits acute, but not subacute, inflammatory pain induced by complete Freund's adjuvant
    Nakamura, Yoki
    Fukushige, Ryo
    Watanabe, Kohei
    Kishida, Yuki
    Hisaoka-Nakashima, Kazue
    Nakata, Yoshihiro
    Morioka, Norimitsu
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2020, 533 (04) : 971 - 975