Separation of peptides by pressurized capillary electrochromatography

被引：0
|
作者：
Zhang, K
Yan, C
Zhang, ZC
Wang, QS
Gao, RY [1 ]
机构：
[1] Nankai Univ, State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China
[2] Unimicro Technol Inc, Pleasanton, CA 94566 USA
来源：
CHINESE CHEMICAL LETTERS | 2003年 / 14卷 / 06期
关键词：
pressurized capillary electrochromatography; peptide; separation; reversed phase; hydrophilic interaction;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel gradient pressurized capillary electrochromatography (pCEC) instrument was developed to separate peptides. Two gradient elution modes, hydrophobic and hydrophilic interaction mode in pCEC, were performed on this instrument. Baseline separation of six peptides was obtained on two gradient modes with C18 column and strong cationic exchange column respectively. The effects of mixer volume and total flow rate of pumps on resolution were also discussed.
引用
收藏
页码：611 / 614
页数：4
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