Separation of Proteins by Gradient Pressurized Capillary Electrochromatography

被引：0
|
作者：
Zhao Liangjuan [1 ]
Zou Juanjuan [3 ]
Wang Xiuli [3 ]
Yan Chao [3 ]
Gao Ruyu [2 ]
Liu Mingyu [4 ]
Lu Xianyu [1 ]
机构：
[1] Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China
[2] Nankai Univ, State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China
[3] Unimicro Shanghai Technol Co Ltd, Shanghai 201203, Peoples R China
[4] China Agr Univ, Beijing 100094, Peoples R China
来源：
CHINESE JOURNAL OF CHROMATOGRAPHY | 2005年 / 23卷 / 06期
关键词：
gradient elution; pressurized capillary electrochromatography; reversed-phase chromatography; proteins;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The separation of four proteins including RNase A, cytochrome C, lysozyme and myoglobin was investigated by reversed-phase gradient pressurized capillary electrochromatography (p-CEC) with 1.5 mu m non-porous silica C-18 stationary phase. This mode was compared with micro-high performance liquid chromatography (mu-HPLC) and the effects of applied voltage, stationary phase and concentration of ion-pairing agent (trifluoroacetic acid, TFA) on the gradient p-CEC were also studied. This separation was performed rapidly on a new CEC instrument Trisep (TM) 2010 GV. The results showed that the retention mechanism of proteins in p-CEC mode is based on both chromatographic partitioning and electrophoretic migration. The results also demonstrated that p-CEC may have great potential for fast and efficient separation of proteins.
引用
收藏
页码：669 / 672
页数：4
相关论文
共 13 条
  • [1] Evaluation of automated isocratic and gradient nano-liquid chromatography and capillary electrochromatography
    Alexander, JN
    Poli, JB
    Markides, KE
    [J]. ANALYTICAL CHEMISTRY, 1999, 71 (13) : 2398 - 2409
  • [2] Colon LA, 2000, ELECTROPHORESIS, V21, P3965, DOI 10.1002/1522-2683(200012)21:18<3965::AID-ELPS3965>3.0.CO
  • [3] 2-T
  • [4] The separation of biomolecules using capillary electrochromatography
    Fu, HJ
    Huang, XD
    Jin, WH
    Zou, HF
    [J]. CURRENT OPINION IN BIOTECHNOLOGY, 2003, 14 (01) : 96 - 100
  • [5] Hachey D L, 2004, REPROD IMMUNOLOGY, V63, P61
  • [6] Role of chromatographic techniques in proteomic analysis
    Neverova, I
    Van Eyk, JE
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 815 (1-2): : 51 - 63
  • [7] Advances in column technology and instrumentation in capillary electrochromatography
    Pyell, U
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2000, 892 (1-2) : 257 - 278
  • [8] Column technology for capillary electrochromatography
    Tang, QL
    Lee, ML
    [J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2000, 19 (11) : 648 - 663
  • [9] Capillary electrochromatography for separation of peptides driven with electrophoretic mobility on monolithic column
    Wu, RA
    Zou, HF
    Ye, ML
    Lei, ZD
    Ni, JY
    [J]. ANALYTICAL CHEMISTRY, 2001, 73 (20) : 4918 - 4923
  • [10] Wu RA, 2002, CHEM J CHINESE U, V23, P213
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