Direct NP- A cost-effective extraction-free RT-qPCR based test for SARS-CoV-2

被引：2
|
作者：
Parikh, Rasesh Y. [1 ]
Nadig, Satish N. [2 ]
Mehrotra, Shikhar [2 ,3 ]
Howe, Philip H.
Gangaraju, Vamsi K. [1 ]
机构：
[1] Med Univ South Carolina, Hollings Canc Ctr, Dept Biochem & Mol Biol, Charleston, SC 29425 USA
[2] Med Univ South Carolina, Hollings Canc Ctr, Dept Surg, Charleston, SC 29425 USA
[3] Med Univ South Carolina, Hollings Canc Ctr, Dept Microbiol & Immunol, Charleston, SC 29425 USA
来源：
HELIYON | 2022年 / 8卷 / 06期
关键词：
COVID-19; SARS-CoV-2; Dualplex; RT-qPCR; Broad-scale; Test;
D O I：
10.1016/j.heliyon.2022.e09735
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Over 2.4 million daily total tests are currently being performed for SARS-CoV-2, in the United States. The most common SARS-CoV-2 tests require RNA extraction and purification. Extraction of RNA is a time-consuming and costly step that requires a constant supply of reagents and accessories. With the current testing demand, the supply chain remains the bottleneck for RNA extraction. Here, we report Direct NP-a cost-effective extraction-free RT-qPCR based dualplex test for SARS-CoV-2 from Nasopharyngeal (NP) swab specimens. Direct NP detects SARS-CoV-2 viral RNA from heat-denatured patient specimens using a dualplex RT-qPCR assay. Direct NP showed 92.5% positive percentage agreement (PPA) (95% Confidence Interval (CI) = 79.61%-98.43%) and 97% negative percent agreement (NPA) (95% CI = 89.11-100%) with the CDC assay. Direct NP reduces the cost per test to $2, making it suitable for broad-scale testing while lowering the cost burden on the healthcare system.
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页数：6
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