The effects of space-travel-relevant radiation doses (gamma radiation) on structure, function, and antioxidant properties of select wheat cultivars (Triticum aestivum L) were investigated. Following radiation treatment, proteins were analyzed using SDS-PAGE, mixograph, and atomic force microscopy methods. Starch granule damage and pasting curve functionality were evaluated; lipid oxidation was determined using a thiobarbituric acid reactive substances method; and antioxidant capacity was measured using 2,2-diphenyl-1-picrythydrazyl analyses. Increasing radiation exposure resulted in significant differences between wheat cultivars. Therefore, low-dose gamma-radiation affects wheat, and differences exist between cultivars, so careful consideration is needed when selecting wheat for use in elevated radiation conditions. The Apogee cultivar is likely a better NASA candidate crop than Perigee; however, Yecora Rojo, Pars hall, and/or Yavaros 79 cultivars may be more stable to radiation and provide better food functionality traits. Ultimately, wheat should be protected from radiation exposure during space travel, if possible, to maintain quality.