Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and Conformation

The pectic polysaccharides extracted from flaxseed (Linum usitatissiumum L.) mucilage and kernel were characterized as rhamnogalacturonan-I (RG-I). In this study, the conformational characteristics of RG-I fractions from flaxseed mucilage and kernel were investigated, using a Brookhaven multi-angle light scattering instrument (batch mode) and a high-performance size exclusion chromatography (HPSEC) system coupled with Viscotek tetra-detectors (flow mode). The M-w of flaxseed mucilage RG-I (FM-R) was 285 kDa, and the structure-sensitive parameter (rho) value of FM-R was calculated as 1.3, suggesting that the FM-R molecule had a star-like conformation. The M-w of flaxseed kernel RG-I (FK-R) was 550 kDa, and the structure-sensitive parameter (rho) values ranged from 0.90 to 1.21, suggesting a sphere to star-like conformation with relatively higher segment density. The correlation between the primary structure and conformation of RG-I was further discussed to better understand the structure-function relationship, which helps the scale-up applications of pectins in food, pharmaceutical, or cosmetic industries.