Detection and identification of a water mixture of E. coli cells and B. subtilis spores with Raman chemical imaging microscopy

被引：0

作者：

Tripathi, Ashish ^{[1
]}

Jabbour, Rabih E. ^{[1
]}

Treado, Patrick J. ^{[2
]}

Neiss, Jason H. ^{[2
]}

Nelson, Matthew P. ^{[2
]}

Jensen, Janet L. ^{[3
]}

Snyder, A. Peter ^{[3
]}

机构：

[1] Sci Applicat Int Corp, POB 68,Gunpowder Branch, Aberdeen Proving Ground, MD 21010 USA

[2] ChemImage Corp, Pittsburgh, PA 15208 USA

[3] Edgewood Chem Biolog Ctr, Aberdeen Proving Ground, MD 21010 USA

来源：

CHEMICAL AND BIOLOGICAL SENSING VIII | 2007年 / 6554卷

关键词：

Raman chemical imaging microspectroscopy; Bacillus atrophaeus; E; coli; principal components analysis; water matrix; biological discrimination; bacteria; recipe tap water;

D O I：

10.1117/12.707788

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Raman spectroscopy is being evaluated as a candidate technology for waterbome pathogen detection and the fidelity of the Raman spectra of microorganisms with respect to their differentiation at the single cell level are investigated. Individual entities are investigated in the microscope field of view (FOV) by Raman chemical imaging microscopy (RCM. The size of a substance was not found to cause spectral confusion when collating individual entities in the FOV by multivariate principal components (PCA) and RCIM methods. Polystyrene (PS) beads in 1-3 micron sizes were collectively grouped together by PCA. Distilled and recipe tap water matrices produced the proper identification of the PS beads throughout the FOV, and all PS beads in a FOV were grouped together by PCA. A mixture of Gram-positive Bacillus atrophaeus spores and Gram-negative E. coli cells were differentiated and distinguished by RCIM.

引用

页数：9