Flipping interferometry and its application for quantitative phase microscopy in a micro-channel

被引：69
|
作者：
Roitshtain, Darina [1 ]
Turko, Nir A. [1 ]
Javidi, Bahram [2 ]
Shaked, Natan T. [1 ]
机构：
[1] Tel Aviv Univ, Fac Engn, Dept Biomed Engn, IL-69978 Tel Aviv, Israel
[2] Univ Connecticut, Dept Elect & Comp Engn, Storrs, CT 06269 USA
来源：
OPTICS LETTERS | 2016年 / 41卷 / 10期
基金：
美国国家科学基金会;
关键词：
COMPACT;
D O I：
10.1364/OL.41.002354
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We present a portable, off-axis interferometric module for quantitative phase microscopy of live cells, positioned at the exit port of a coherently illuminated inverted microscope. The module creates on the digital camera an interference pattern between the image of the sample and its flipped version. The proposed simplified module is based on a retro-reflector modification in an external Michelson interferometer. The module does not contain any lenses, pinholes, or gratings and its alignment is straightforward. Still, it allows full control of the off-axis angle and does not suffer from ghost images. As experimentally demonstrated, the module is useful for quantitative phase microscopy of live cells rapidly flowing in a micro-channel. (C) 2016 Optical Society of America
引用
收藏
页码：2354 / 2357
页数：4
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