Elastic volume reconstruction from series of ultra-thin microscopy sections

被引：0

作者：

Saalfeld, Stephan ^{[1
]}

Fetter, Richard ^{[2
]}

Cardona, Albert ^{[2
,3
,4
]}

Tomancak, Pavel ^{[1
]}

机构：

[1] Max Planck Inst Mol Cell Biol & Genet, Dresden, Germany

[2] Howard Hughes Med Inst, Ashburn, VA USA

[3] Univ Zurich, Inst Neuroinformat, Zurich, Switzerland

[4] ETH, Zurich, Switzerland

来源：

NATURE METHODS | 2012年 / 9卷 / 07期

关键词：

IMAGE; REGISTRATION;

D O I：

10.1038/NMETH.2072

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Anatomy of large biological specimens is often reconstructed from serially sectioned volumes imaged by high-resolution microscopy. We developed a method to reassemble a continuous volume from such large section series that explicitly minimizes artificial deformation by applying a global elastic constraint. We demonstrate our method on a series of transmission electron microscopy sections covering the entire 558-cell Caenorhabditis elegans embryo and a segment of the Drosophila melanogaster larval ventral nerve cord.

引用

页码：717 / U280

页数：7

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