Continuous biosynthesis of geranyl butyrate in a circulating fluidized bed reactor

被引:10
|
作者
Wang, Lu [1 ]
Chen, Gang [1 ]
Tang, Jing [1 ]
Ming, Miao [1 ]
Jia, Chengsheng [1 ]
Feng, Biao [1 ]
机构
[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Lihu Rd 1800, Wuxi 214122, Jiangsu, Peoples R China
关键词
CRL; Immobilization; Geranyl butyrate; CFBR; CANDIDA-RUGOSA LIPASE; ENZYMATIC-SYNTHESIS; IMMOBILIZED LIPASES; ACETATE; GLYCERIDES; LAURATE; ESTERS; OIL;
D O I
10.1016/j.fbio.2018.05.007
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The continuous synthesis of geranyl butyrate in organic solvent was studied. The Candida rugosa lipase(CRL) was firstly immobilized on weak-acidic macroporous cation exchange resin D151. The batch experiments indicated that the adequate acid/alcohol molar ratio was 1:1.5 and the reaction time was 3.5-4 h. Then the continuous synthesis was realized with immobilized Candida rugosa lipase in a circulating fluidized bed reactor(CFBR). A stable ester yield of 77% was achieved under optimal conditions: enzyme loading 8 g, expansion ratio 1.6, feed flow rate 0.07 ml/min and butyric acid concentration 75 mmol/l. The results provide the basis for the scale-up of the process.
引用
收藏
页码:60 / 65
页数:6
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