Genotyping Neisseria gonorrhoeae gyrA and penA antimicrobial genes from remnant Neisseria gonorrhoeae positive Cepheid Xpert® clinical specimens - A feasibility study

被引：0
|
作者：
Qadir, S. [1 ]
Ellis, O. [2 ]
Keizur, E. M. [1 ]
Stafylis, C. [1 ]
Cortado, R. [3 ]
Klausner, J. D. [1 ]
机构：
[1] Univ Calif Los Angeles, David Geffen Sch Med, Div Infect Dis, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Fielding Sch Publ Hlth, Dept Environm Hlth Sci, Los Angeles, CA USA
[3] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pediat, Los Angeles, CA 90095 USA
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2020年 / 168卷
基金：
美国国家卫生研究院;
关键词：
Genotyping; penA; gyrA; Neisseria gonorrhoeae; Antibiotic resistance; TIME PCR ASSAY; RESISTANCE;
D O I：
10.1016/j.mimet.2019.105783
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Neisseria gonorrhoeae (NG) has developed resistance to most antibiotics, making it increasingly difficult to treat. Previous studies have predicted antimicrobial NG susceptibility based on the antimicrobial gene target DNA gyrase subunit A (gyrA) codon serine 91 and the penicillin-binding protein 2 (penA) using Roche Cobas (R) and Hologic APTIMA (TM) clinical specimens. We studied whether similar methods could be used on remnant NG-positive Cepheid Xpert (R) specimens.
引用
收藏
页数：2
相关论文
共 50 条
  • [31] Molecular Assay for Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae Isolates from Cultures and Clinical Nucleic Acid Amplification Test Specimens
    Peterson, S. W.
    Martin, I.
    Demczuk, W.
    Bharat, A.
    Hoang, L.
    Wylie, J.
    Allen, V.
    Lefebvre, B.
    Tyrrell, G.
    Horsman, G.
    Haldane, D.
    Garceau, R.
    Wong, T.
    Mulvey, M. R.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2015, 53 (11) : 3606 - 3608
  • [32] Antimicrobial resistance in Neisseria gonorrhoeae and Mycoplasma genitalium isolates from the private healthcare sector in South Africa: A pilot study
    Maduna, L. D.
    Peters, R. P. H.
    Kingsburgh, C.
    Strydom, K-A
    Kock, M. M.
    SAMJ SOUTH AFRICAN MEDICAL JOURNAL, 2021, 111 (10): : 995 - 997
  • [33] Review of use of a new rapid real-time PCR, the Cepheid GeneXpert® (Xpert) CT/NG assay, for Chlamydia trachomatis and Neisseria gonorrhoeae: results for patients while in a clinical setting
    Gaydos, Charlotte A.
    EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2014, 14 (02) : 135 - 137
  • [34] In-vitro antimicrobial activity of HSR-903, a new fluoroquinolone, against clinical isolates of Neisseria gonorrhoeae with quinolone resistance-associated alterations in GyrA and ParC
    Deguchi, T
    Yasuda, M
    Ishihara, S
    Takahashi, Y
    Okezaki, E
    Nagata, O
    Saito, I
    Kawada, Y
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1997, 40 (03) : 437 - 439
  • [35] Comparison of in vitro antimicrobial activity of AM-1155 with those of tosufloxacin and fleroxacin against clinical isolates of Neisseria gonorrhoeae harboring quinolone resistance alterations in GyrA and ParC
    Deguchi, T
    Yasuda, M
    Nakano, M
    Ozeki, S
    Kanematsu, E
    Fukuda, H
    Maeda, S
    Saito, I
    Kawada, Y
    CHEMOTHERAPY, 1997, 43 (04) : 239 - 244
  • [36] Mismatch Amplification Mutation Assay-Based Real-Time PCR for Rapid Detection of Neisseria gonorrhoeae and Antimicrobial Resistance Determinants in Clinical Specimens
    Dona, Valentina
    Smid, Joost H.
    Kasraian, Sara
    Egli-Gany, Dianne
    Dost, Ferah
    Imeri, Fatime
    Unemo, Magnus
    Low, Nicola
    Endimiani, Andrea
    JOURNAL OF CLINICAL MICROBIOLOGY, 2018, 56 (09)
  • [37] Neisseria gonorrhoeae diagnostic escape from a gyrA-based test for ciprofloxacin susceptibility and the effect on zoliflodacin resistance: a bacterial genetics and experimental evolution study
    Rubin, Daniel H. F.
    Mortimer, Tatum
    Grad, Yonatan H.
    LANCET MICROBE, 2023, 4 (04): : e247 - e254
  • [38] THE ACCEPTABILITY OF SELF-SAMPLING AT HOME FOR CHLAMYDIA TRACHOMATIS AND NEISSERIA GONORRHOEAE IN MEN AND WOMEN; RESULTS FROM THE FEASIBILITY STUDY TO DETERMINE THE TIME TAKEN FOR NAATS TESTS TO BECOME NEGATIVE FOLLOWING TREATMENT FOR CHLAMYDIA TRACHOMATIS AND NEISSERIA GONORRHOEAE IN MEN AND WOMEN
    Sultan, Binta
    Oakland, Clare
    Brima, Nataliya
    Patel, Hemi
    Schembri, Gabriel
    Ison, Cathy
    Benn, Paul
    SEXUALLY TRANSMITTED INFECTIONS, 2016, 92 : A87 - A87
  • [39] RECOVERY OF NEISSERIA-GONORRHOEAE FROM CLINICAL SPECIMENS AFTER DIRECT CULTURE AT 25-DEGREES-C AND 28-DEGREES-C
    ANNEAR, DI
    BLUMS, M
    ISMAIL, A
    JOURNAL OF CLINICAL PATHOLOGY, 1982, 35 (08) : 902 - 903
  • [40] COMPARISON OF MODIFIED NEW-YORK-CITY MEDIUM WITH MARTIN-LEWIS MEDIUM FOR RECOVERY OF NEISSERIA-GONORRHOEAE FROM CLINICAL SPECIMENS
    GRANATO, PA
    PAEPKE, JL
    WEINER, LB
    JOURNAL OF CLINICAL MICROBIOLOGY, 1980, 12 (06) : 748 - 752
12345